Reactions of arsine with hemoglobin.
Reactions of arsine with hemoglobin.
复制标题
砷化氢与血红蛋白的反应。
DOI：
10.1080/009841096161852
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
D. E. Carter
中科院分区：
文献类型：
--
作者：
K. Hatlelid;C. Brailsford;D. E. Carter
用大鼠和狗的红细胞（RBC）研究了砷化氢（AsH_3）诱导溶血的机制。AsH3诱导的狗红细胞溶血完全阻断一氧化碳（CO）预孵育和减少纯氧（O2）相比，在空气中孵育。由于CO和O2与血红素结合并减少溶血，这些结果表明AsH3与血红素配体结合口袋中的血红蛋白或与血红素铁之间发生反应。此外，亚硝酸钠诱导高铁血红蛋白（metHb）的85%和34%的总血红蛋白，否则完整的红细胞导致56%和16%的溶血减少，分别孵育4小时后。这为血红蛋白参与AsH3诱导的溶血机制提供了额外的证据。研究了在纯化的狗血红蛋白溶液中AsH_3与血红蛋白的反应。AsH3与各种纯化的血红蛋白物种的反应的分光光度法研究表明，AsH3与HbO2反应，产生metHb，并最终，降解血红蛋白的特征在于总沉淀的蛋白质。AsH3没有改变脱氧血红蛋白的光谱，并没有导致在氧气中的metHb的降解，但绑定到和还原metHb在没有氧气。这些数据表明，AsH3与氧合血红蛋白HbO2的反应可能导致溶血，但AsH3与metHb之间存在可能不直接参与溶血过程的反应。
The mechanism of arsine (AsH3) induced hemolysis was studied in vitro using isolated red blood cells (RBCs) from the rat or dog. AsH3-induced hemolysis of dog red blood cells was completely blocked by carbon monoxide (CO) preincubation and was reduced by pure oxygen (O2) compared to incubations in air. Since CO and O2 bind to heme and also reduce hemolysis, these results suggested a reaction between AsH3 and hemoglobin in the heme-ligand binding pocket or with the heme iron. Further, sodium nitrite induction of methemoglobin (metHb) to 85% and 34% of total Hb in otherwise intact RBCs resulted in 56% and 16% decreases in hemolysis, respectively, after incubation for 4 h. This provided additional evidence for the involvement of hemoglobin in the AsH3-induced hemolysis mechanism. Reactions between AsH3 and hemoglobin were studied in solutions of purified dog hemoglobin. Spectrophotometric studies of the reaction of AsH3 with various purified hemoglobin species revealed that AsH3 reacted with HbO2 to produce metHb and, eventually, degraded Hb characterized by gross precipitation of the protein. AsH3 did not alter the spectrum of deoxyHb and did not cause degradation of metHb in oxygen, but bound to and reduced metHb in the absence of oxygen. These data indicate that a reaction of AsH3 with oxygenated hemoglobin HbO2, may lead to hemolysis, but there are reactions between AsH3 and metHb that may not be directly involved in the hemolytic process.