Gut Microbiota Richness and Diversity Track With T Cell Aging in Healthy Adults.
Gut Microbiota Richness and Diversity Track With T Cell Aging in Healthy Adults.
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健康成人肠道微生物群丰富度和多样性与 T 细胞衰老的关系。
DOI：
10.1093/gerona/glad276
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Kiecolt-Glaser,JaniceK
中科院分区：
文献类型：
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作者：
Madison,AnneliseA;Burd,ChristinE;Andridge,Rebecca;Wilson,StephanieJ;Bailey,MichaelT;Belury,MarthaA;Spakowicz,DanielJ;Malarkey,WilliamB;Kiecolt-Glaser,JaniceK
背景本研究探讨了肠道微生物群的多样性和丰富性如何与96名所有年龄段的健康成年人的T细胞老化。它还探讨了这些链接是否不同的整个lifes.MethodsPeripheral血液从96研究参与者（N =96，年龄21-72），以评估T细胞老化（p16 ink 4a，p14 ARF，B3 gat 1，Klrg 1）和DNA甲基化的mRNA标记。 将T细胞衰老mRNA标记物组合成衰老指数，并使用Horvath表观遗传时钟算法基于超过500个基因座的DNA甲基化状态来计算表观遗传年龄。参与者还收集了粪便样本，对16 S rRNA基因的V4区域进行测序，以获得Shannon和Simpson多样性指数，以及观察到的操作分类单位（丰富度）的总数。模型控制BMI，合并症，性别，饮食质量，吸烟状况，体力活动，和睡眠quality.ResultsLower微生物群丰富度与较高的T细胞年龄的基础上的mRNA标记，但当探测区域的显着性，这种关系仅在45岁及以上的成年人显着（p = 0.03）。 较低的香农多样性（p = 0.05）和丰富性（p = 0.07）与较高的表观遗传年龄（即，更大的T细胞DNA甲基化）轻微相关。 这些结果表明肠道微生物组和免疫衰老之间的相互作用，需要进一步的实验工作。
BackgroundThis study examined how gut microbiota diversity and richness relate to T cell aging among 96 healthy adults of all ages. It also explored whether these links differed throughout the lifespan.MethodsPeripheral blood was obtained from 96 study participants (N =96, aged 21–72) to assess mRNA markers of T cell aging (p16ink4a, p14ARF, B3gat1, Klrg1) and DNA methylation. T cell aging mRNA markers were combined into an aging index, and the Horvath epigenetic clock algorithm was used to calculate epigenetic age based on DNA methylation status of over 500 loci. Participants also collected a stool sample from which the V4 region of the 16S rRNA gene was sequenced to derive the Shannon and Simpson diversity indices, and the total count of observed operational taxonomic units (richness). Models controlled for BMI, comorbidities, sex, dietary quality, smoking status, physical activity, and sleep quality.ResultsLower microbiota richness was associated with higher T cell age based on mRNA markers, but when probing the region of significance, this relationship was only significant among adults 45 years and older (p =.03). Lower Shannon diversity (p =.05) and richness (p =.07) marginally correlated with higher epigenetic age (ie, greater T cell DNA methylation).ConclusionsGut microbiota complexity may correspond with the rate of T cell aging, especially in mid-to-late life. These results suggest an interplay between the gut microbiome and immunological aging that warrants further experimental work.