Effects of dexamethasone on the morphology, gene expression and hepatic histology in adult female mosquitofish (Gambusia affinis)
Effects of dexamethasone on the morphology, gene expression and hepatic histology in adult female mosquitofish (Gambusia affinis)
复制标题
地塞米松对成年雌性蚊鱼（Gambusia affinis）形态、基因表达和肝组织学的影响
DOI：
10.1016/j.chemosphere.2021.129797
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Yao Li
中科院分区：
文献类型：
--
作者：
Zhong Lishan;Liang Yan-Qiu;Lu Mixue;Pan Chang-Gui;Dong Zhongdian;Zhao Hui;Li Chengyong;Lin Zhong;Yao Li
糖皮质激素(GC)包括天然激素和合成化学物质，可对鱼类的生理性能、发育和繁殖产生影响。地塞米松(DEX)是一种人工合成的糖皮质激素，广泛用作药物，通常存在于不同浓度的流出物中。用0、0.5、5和50μg/L浓度的地塞米松处理成年雌性亲缘蚊虫60天。为了评价地塞米松对蚊虫的影响，研究了蚊体的肛鳍和骨骼的形态参数、mRNA表达丰度和肝脏的组织学变化。结果表明，地塞米松增加了肛鳍3号射线的切面数，降低了16L、15D和16D的骨骼参数，提示地塞米松有可能导致弱雄性化。此外，地塞米松显著下调α、ARβ、ERβ、VTGC和CYP19A基因的转录表达水平，这可能是导致雌性雄性化弱的原因之一。此外，地塞米松对小鼠肝组织也有一定的损伤作用。综上所述，这项研究表明，被DEX污染的水环境在种群水平上对蚊虫产生了负面影响。
Glucocorticoids (GCs), including natural hormones as well as synthetic chemicals, can pose influences on physiological performance, development and reproduction of fish. Dexamethasone (DEX) is a synthetic glucocorticoid widely used as pharmaceutical and usually exists in effluents with varying degrees of concentrations. In this study, adult female mosquitofish (Gambusia affinis) were treated by DEX at concentrations of 0, 0.5, 5 and 50 μg/L for 60 days. Morphological parameters of anal fin and skeleton, mRNA expression abundance, and histological alterations of liver were investigated to assess effects of DEX on mosquitofish. The results showed that DEX increased number of sections of ray 3 in anal fin and decreased 16L, 15D and 16D in skeletal parameters, which indicates DEX could potentially lead to weak masculinization. Furthermore, transcriptional expression levels ofARα,ARβ,ERβ,VTGC and CYP19Agenes were notably down-regulated by DEX, which will contribute to weak masculinization in females. In addition, the damage to liver tissue was also induced by DEX. Taken together, this research demonstrated that aquatic environments contaminated by DEX have negative effects on mosquitofish at a population level.