Size-Dependent Optical Absorption of Layered MoS2 and DNA Oligonucleotides Induced Dispersion Behavior for Label-Free Detection of Single-Nucleotide Polymorphism
Size-Dependent Optical Absorption of Layered MoS2 and DNA Oligonucleotides Induced Dispersion Behavior for Label-Free Detection of Single-Nucleotide Polymorphism
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层状 MoS2 和 DNA 寡核苷酸的尺寸依赖性光学吸收诱导的分散行为，用于单核苷酸多态性的无标记检测
DOI：
10.1002/adfm.201500180
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发表时间：
2015-06-17
影响因子：
19
通讯作者：
Li, Nian Bing
中科院分区：
文献类型：
--
作者：
Li, Bang Lin;Zou, Hao Lin;Li, Nian Bing
研究了半导体（2H）层状二硫化钼（MoS2）对单链和双链DNA （ssDNA）和（dsDNA）的光学吸收。在高浓度盐的存在下，层状二硫化钼呈快速聚集趋势，导致纳米片的纵向和横向尺寸增大，这是范德华引力和电双层斥力相互作用的结果。同时，DNA寡核苷酸的协同作用显著抑制了层状二硫化钼的聚集行为。ssDNA可以吸附在层状MoS2表面，即使在高浓度盐存在的情况下也具有很大的分散性，而当ssDNA被dsDNA取代时，分散性减弱。而与带隙能量为零的石墨烯相比，具有半导体性质的层状二硫化钼在可见波长区域表现出良好的特征光吸收，致力于探索层状二硫化钼的聚集行为。因此，DNA寡核苷酸诱导层状二硫化钼的大小控制，有助于其在可见光区特征吸收的规律变化，被认为是一种检测单核苷酸多态性的无标记生物测定方法。该方法操作简便，特异性高，具有广阔的应用前景。
Size-dependent optical absorption of semiconductive (2H) layered molybdenum disulfide (MoS2), exhibiting great discrimination abilities to single- and double-stranded DNA (ssDNA) and (dsDNA), is studied. In the presence of high concentration of salt, layered MoS2 trends to aggregate rapidly, leading to the increases of sizes in both vertical and lateral dimensions of the nanosheets, which results from the interplay between van der Waals attraction and electrical double-layer repulsion. Meanwhile, the aggregation behavior of layered MoS2 is remarkably inhibited by the synergistic effects of DNA oligonucleotides. ssDNA can adsorb on the surface of layered MoS2, resulting in a great dispersion, even in the presence of high concentration of salt, while the dispersion behavior is weakened when ssDNA is replaced by dsDNA. Whereas compared to graphene with zero bandgap energy, layered MoS2, with semiconductive properties, exhibits great characteristic optical absorption in visible wavelength region devoted to exploring the aggregation behavior of layered MoS2. Therefore, DNA oligonucleotides induced size control of layered MoS2, contributing to the regular change of its characteristic absorption in visible region, is considered a label-free bioassay for the detection of single-nucleotide polymorphism. Due to its easy operation and high specificity, it is expected that the proposed assay holds great promise for further applications.