Involvement of sensory neuron in bone defect repair in rats
Involvement of sensory neuron in bone defect repair in rats
复制标题
感觉神经元参与大鼠骨缺损修复
DOI：
10.1093/jmicro/dfr075
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发表时间：
2011
影响因子：
1.8
通讯作者：
他
中科院分区：
文献类型：
--
作者：
Henmi A;Nakamura M;他
我们研究了感觉失神经大鼠的骨修复，并与对照组进行了比较，以阐明感觉神经元的参与。九周龄雄性Wistar大鼠皮下注射辣椒素去神经感觉神经元。用等量载药处理的大鼠作为对照。在顶骨上形成了标准化的骨缺损。用x线定量分析测定修复骨量，用实时聚合酶链反应（real-time polymerase chain reaction， PCR）测定骨钙素和组织蛋白酶K mRNA表达量。定量x线分析显示，在任何时间点，辣椒素处理组的骨修复量的标准差和变异系数都远高于对照组，这意味着辣椒素处理组的骨修复量的个体差异比对照组大。此外，仅在辣椒素处理组中，x线片显示标准化缺损周围已有骨的放射透光，组织学观察显示放射透光区域对应一些多核细胞。Real-time PCR结果显示，对照组与辣椒素处理组骨钙素和组织蛋白酶K mRNA表达量无显著差异。这些结果提示辣椒素诱导的感觉去神经支配影响骨缺损的修复。
We investigated bone repair in sensory-denervated rats, compared with controls, to elucidate the involvement of sensory neurons. Nine-week-old male Wistar rats received subcutaneous injections of capsaicin to denervate sensory neurons. Rats treated with the same amount of vehicle served as controls. A standardized bone defect was created on the parietal bone. We measured the amount of repaired bone with quantitative radiographic analysis and the mRNA expressions of osteocalcin and cathepsin K with real-time polymerase chain reaction (PCR). Quantitative radiographic analysis showed that the standard deviations and coefficients of variation for the amount of repaired bone were much higher in the capsaicin-treated group than in the control group at any time point, which means that larger individual differences in the amount of repaired bone were found in capsaicin-treated rats than controls. Furthermore, radiographs showed radiolucency in pre-existing bone surrounding the standardized defect only in the capsaicin-treated group, and histological observation demonstrated some multinuclear cells corresponding to the radiolucent area. Real-time PCR indicated that there was no significant difference in the mRNA expression levels of osteocalcin and cathepsin K between the control group and the capsaicin-treated group. These results suggest that capsaicin-induced sensory denervation affects the bone defect repair.