Fibroblast-like synoviocyte-chondrocyte interaction in cartilage degradation.
Fibroblast-like synoviocyte-chondrocyte interaction in cartilage degradation.
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DOI：
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发表时间：
2007
影响因子：
3.7
通讯作者：
M. Steenvoorden;Ruud A. Bank;H. Ronday;R. Toes;T. Huizinga;Jeroen DeGroot
中科院分区：
文献类型：
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作者：
M. Steenvoorden;Ruud A. Bank;H. Ronday;R. Toes;T. Huizinga;Jeroen DeGroot
目的关节疾病的体外模型通常集中于单一细胞类型，如骨关节炎（OA）中的软骨细胞或类风湿关节炎（RA）中的成纤维细胞样滑膜细胞（滑膜细胞）。然而，这些关节疾病影响整个关节，软骨细胞和滑膜细胞之间的相互作用可能在疾病病理学中起重要作用。本研究旨在研究藻酸盐回收软骨细胞法作为软骨降解模型的使用，并研究软骨细胞和滑膜细胞之间的相互作用。方法牛软骨细胞在藻酸盐微球中培养1周后，取出软骨球接种于transwell中。每两天分析软骨切片的蛋白聚糖含量（比色法，Blyscan GAG试剂盒）、胶原蛋白含量（HPLC）和胶原蛋白HP和LP交联（HPLC）。对于降解实验，用IL-1 β或TNF-α刺激用（35）S标记的软骨切片的单一培养物和与滑膜细胞的共培养物。7天后，测量（35）S释放作为软骨降解的量度。结果经生化分析后，取3周龄软骨样切片进行软骨降解实验。滑膜细胞能够诱导软骨降解，只有在活的软骨细胞的存在下。此外，细胞因子白细胞介素1（IL-1 β）和肿瘤坏死因子（TNF-α）只能通过软骨细胞而不是滑膜细胞诱导软骨降解。结论藻酸盐修复软骨细胞的方法为研究滑膜细胞与软骨细胞之间的相互作用提供了一种新的软骨降解模型。
OBJECTIVE In vitro models for joint diseases often focus on a single cell type, such as chondrocytes in osteoarthritis (OA) or fibroblast-like synoviocytes (synoviocytes) in rheumatoid arthritis (RA). However, these joint diseases affect the whole joint and interaction between chondrocytes and synoviocytes may play an important role in disease pathology. The current study was designed to study the use of the alginate recovered chondrocyte method as a model for cartilage degradation and to study interaction between chondrocytes and synoviocytes. METHODS Bovine chondrocytes were cultured in alginate beads for 1 week, subsequently chondrons were retrieved and seeded into transwells. Every two days cartilage-slices were analysed for proteoglycan content (colorimetric, Blyscan GAG kit), collagen content (HPLC) and collagen HP and LP crosslinking (HPLC). For degradation experiments, monocultures of cartilage-slices labelled with (35)S and cocultures with synoviocytes were stimulated with IL-1beta or TNF-alpha. After 7 days, (35)S release was measured taken as a measure of cartilage degradation. RESULTS After biochemical analysis, three week old cartilage-like slices were chosen to perform cartilage-degradation experiments. Synoviocytes were able to induce cartilage degradation only in the presence of living chondrocytes. In addition, the cytokines interleukin 1 (IL-1beta) and tumor necrosis factor (TNF-alpha) were only able to induce cartilage degradation by chondrocytes, not by synoviocytes. CONCLUSION These data indicate that the alginate recovered chondrocyte method provides a novel model for cartilage degradation in which the interaction between synoviocytes and chondrocytes can be studied.