CO bond angle changes in photolysis of carboxymyoglobin.
CO bond angle changes in photolysis of carboxymyoglobin.
复制标题
碳氧肌红蛋白光解过程中CO键角的变化。
DOI：
10.1021/bi00318a021
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Chance,B
中科院分区：
文献类型：
--
作者：
Powers,L;Sessler,JL;Woolery,GL;Chance,B
材料和方法样品制备、低温技术和样品监测。 Ni (CO) 4 、Na 2 Fe (CO) 4 和Fe (CO) 5 是商业上购买的最高纯度的，无需进一步纯化即可使用。 Fe (TPP)(Py)(CO)采用Peng & Ibers (1976)的方法制备，“袖珍”卟啉FePocPiv (1-Melm)(CO)采用Coliman等人(1983a)的方法制备，“栅栏”卟啉FeTpivP (l-MeIm)(CO)采用Coliman等人(1975)的方法制备。 MbO 2 、met-Mb和HbO 2 通过与报道的MbCO相似的方法制备(Chance等人，1983)。低温技术和通过光谱法进行的样品监测与 MbCO 详细报道的技术和样品监测相同（Chanceet al., 1983）。数据分析。数据通过先前讨论的程序进行分析（Powers 等人，1981；Leeet 等人，1981；Chance 等人，1983），包括“孤立原子”贡献的背景扣除和 kl（波矢量）乘法，然后进行傅里叶变换。各自的配位壳贡献通过傅立叶滤波器和反变换来分离。
Materials and MethodsSample Preparation, Cryogenic Technique, and Sample Monitoring. Ni (CO) 4, Na2Fe (CO) 4, and Fe (CO) 5 were purchased commercially at the highest purity available and used without further purification. Fe (TPP)(Py)(CO) was prepared by the method of Peng & Ibers (1976), the “pocket” porphyrin FePocPiv (1-Melm)(CO) by the method of Coliman et al.(1983a), and the “picket fence” porphyrin FeTpivP (l-MeIm)(CO) by the method of Coliman et al.(1975). Mb02, met-Mb, and Hb02 were prepared by methods similar to those reported for MbCO (Chance et al., 1983). Cryogenic techniques and sample monitoring by optical spectroscopy were identical with those reportedin detail for MbCO (Chanceet al., 1983).Data Analysis. Data were analyzed by procedures discussed previously (Powers et al., 1981; Leeet al., 1981; Chance et al., 1983) and included background subtraction of the “isolated atom” contribution and kl (wave vector) multiplication fol-lowed by Fourier transformation. The respective coordination shell contributions were isolated by Fourier filter and back-transformation.