Resistance to Permethrin in Aedes aegypti (L.) in Northern Mexico
Resistance to Permethrin in Aedes aegypti (L.) in Northern Mexico
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DOI：
10.3958/059.034.0207
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发表时间：
2009-06-01
影响因子：
0.4
通讯作者：
Ponce Garcia, Gustavo
中科院分区：
文献类型：
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作者：
Flores, Adriana E.;Reyes Solis, Guadalupe;Ponce Garcia, Gustavo
对墨西哥北部科阿韦拉州、索诺拉州和塔毛利帕斯州的埃及伊蚊进行氯菊酯诊断剂量测定。将10组每100只雌性暴露于获得的DID并产生50%的死亡率后，将个体分为两类：幸存者和死亡。对每一类蚊子进行解剖，将其头部、胸部和腹部分开。在头部和胸部进行生化试验，以确定抗性相关酶的活性，包括：a和p酯酶、混合功能氧化酶（MFOs）、谷胱甘肽- s转移酶（GST）和不敏感乙酰胆碱酯酶（iAChE）。结果与一株伊蚊敏感株进行了比较。来自新奥尔良的埃及语。Coahuila的一个种群显示iAChE是唯一超过敏感菌株设定的阈值的酶活性，然而，这种机制与对拟除虫菊酯的抗性无关，而是与对有机磷和氨基甲酸酯类杀虫剂的抗性有关。对于来自塔毛利帕斯州的种群，所研究的机制都不是给予氯菊酯抗性的重要机制。在索诺拉州索诺伊塔的人群中，mfo以较高的活动水平存在，似乎是主要的解毒机制。
Diagnostic dose (DD) of permethrin was determined in Aedes aegypti (L.), from three states (Coahuila, Sonora, and Tamaulipas) in northern Mexico. After exposing 10 groups of 100 females to the DID obtained and producing 50% mortality, individuals were divided into two categories: survivors and dead. Mosquitoes in each of these categories were dissected to separate the head, thorax, and abdomen. Biochemical tests were done on the head and thorax to determine activity by resistance-related enzymes including: a and P-esterases, mixed-function oxidases (MFOs), glutathione-S-transferase (GST) and insensitive acetyl cholinesterase (iAChE). Results were compared with those for a susceptible strain of Ae. aegrypti from New Orleans. A population from Coahuila showed iAChE as the only enzyme activity that surpassed the threshold established with the susceptible strain, however, this mechanism is not associated with resistance to pyrethroids, but rather with resistance to organophosphate and carbamate insecticides. For the populations from Tamaulipas, none of the mechanisms studied were important in conferring resistance to permethrin. MFOs were present at elevated levels of activity, appearing as the main detoxifying mechanism, in the population from Sonoita, Sonora state.