Apolipoprotein J/clusterin expression defines distinct stages of blastocyst implantation in the mouse uterus.
Apolipoprotein J/clusterin expression defines distinct stages of blastocyst implantation in the mouse uterus.
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DOI：
10.1095/biolreprod55.4.740
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发表时间：
1996-10
影响因子：
3.6
通讯作者：
T. L. Brown;B. C. Moulton;D. Witte;D. Swertfeger;J. Harmony
中科院分区：
文献类型：
--
作者：
T. L. Brown;B. C. Moulton;D. Witte;D. Swertfeger;J. Harmony
子宫内膜是一个动态的组织，对激素和生长因子做出反应，以适应，调节和培育发育中的胚胎。为了提供线索的分子机制的基础上的反应，这一组织，我们已经开始确定基因表达在特定阶段的早期妊娠。载脂蛋白J（apoJ）基因编码一种分泌性糖蛋白，能够结合脂质和膜活性蛋白。子宫apoJ基因活性没有检测到受精后立即，但腺上皮表达的apoJ mRNA出现之前的时间胚泡植入和持续植入后。在植入过程中，子宫腔上皮细胞也表达apoJ，但表达被排除在邻近的附着囊胚的网站管腔细胞。ApoJ蛋白在腺和子宫腔中积累，接近表达apoJ mRNA的上皮细胞。我们认为apoJ的表达是子宫接受胚泡着床的一个标志。随后在子宫间质细胞类型和环形肌肌细胞中apoJ信息的表达与蜕膜化的发生相一致。在此期间，纵向肌肉层的肌细胞没有显示出apoJ mRNA的证据。ApoJ蛋白定位于非蜕膜化组织，但在蜕膜化细胞中不明显。相反，蛋白质分散在整个环形和纵向子宫肌层。在子宫内的脂肪处理的女性刺激油，apoJ也表达在蜕膜化的基质细胞和环形肌细胞，表明信号专门从胚胎本身传输不负责apoJ mRNA的积累。
The endometrium is a dynamic tissue that responds to hormonal cues and growth factors to accommodate, regulate, and nurture developing embryos. To provide clues about the molecular mechanisms underlying the responsiveness of this tissue, we have begun to identify genes that are expressed at specific stages of early pregnancy. One such gene, apolipoprotein J (apoJ), encodes a secretory glycoprotein capable of binding lipids and membrane-active proteins. Uterine apoJ gene activity was not detected immediately following fertilization, but glandular epithelial expression of apoJ mRNA appeared just before the time of blastocyst implantation and persisted postimplantation. During implantation, uterine luminal epithelial cells also expressed apoJ, but expression was excluded from luminal cells adjacent to the sites of attached blastocysts. ApoJ protein accumulated in the glandular and uterine lumens in proximity to the epithelial cells that expressed apoJ mRNA. We suggest that apoJ expression is a marker of uterine receptivity to blastocyst implantation. Subsequent expression of apoJ message in uterine stromal cell types and in circular muscle myocytes coincided with the onset of decidualization. During this period the myocytes of the longitudinal muscle layer showed no evidence of apoJ mRNA. ApoJ protein was localized to nondecidualized tissue but was not evident in decidualized cells. In contrast, the protein was dispersed throughout both the circular and longitudinal myometrium. In the uteri of hormone-treated females stimulated with oil, apoJ was also expressed during decidualization in stromal cells and in circular myocytes, indicating that signals specifically transmitted from the embryo itself are not responsible for apoJ mRNA accumulation.