Detection of nifH Sequences in sugarcane (Saccharum officinarum L.) and pineapple (Ananas comosus [L.] Merr.)
Detection of nifH Sequences in sugarcane (Saccharum officinarum L.) and pineapple (Ananas comosus [L.] Merr.)
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DOI：
10.1111/j.1747-0765.2005.tb00034.x
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发表时间：
2005-04-01
影响因子：
2
通讯作者：
Yoneyama, T
中科院分区：
文献类型：
--
作者：
Ando, S;Goto, M;Yoneyama, T
为了在没有培养方法的情况下检测植物中的n -2固定细菌，从甘蔗（Saccharum officinarum L.）茎和菠萝（Ananas comosus [L.）叶中提取DNA，用退化引物扩增nifH基因片段。)稳定)。nifH克隆的序列与慢生根瘤菌属、沙雷氏菌属和克雷伯氏菌属的序列同源。与这三个属同源的nifH序列可能是甘蔗和菠萝内生重氮营养菌的优势菌株，因为这些序列通常在不同寄主植物的不同地点采集的样品中检测到。另一方面，在甘蔗中未检测到与重氮营养菌（Gluconacetobacter diazotrophicus）相关的nifH序列。重氮营养菌是一种内生固氮细菌，此前已从甘蔗中分离到。这表明在本研究中使用的甘蔗植株中不存在重氮营养菌或存在少量重氮营养菌。
To detect N-2-fixing bacteria in a plant without using culture methods, nifH gene segments were amplified with degenerate primers from DNA extracted from stems of sugarcane (Saccharum officinarum L.) and leaves of pineapple (Ananas comosus [L.] Merr.). Sequences of the nifH clones were homologous to those of bacteria in the genera Bradyrhizobium, Serratia, and Klebsiella. The bacteria with nifH sequences homologous to those of the three genera may be promising candidates for predominant endophytic diazotrophs in sugarcane and pineapple, because these sequences were commonly detected in samples from different host plants, which were collected at different locations. On the other hand, no nifH sequence related to Gluconacetobacter diazotrophicus, which is an endophytic N-2-fixing bacterium and had been previously isolated from sugarcane, was detected in sugarcane. This indicates the absence or the presence of few G. diazotrophicus in the stems of the sugarcane plants used in the current study.