LIQUID-CHROMATOGRAPHIC ANALYSIS OF MESNA AND DIMESNA IN PLASMA AND URINE OF PATIENTS TREATED WITH MESNA
LIQUID-CHROMATOGRAPHIC ANALYSIS OF MESNA AND DIMESNA IN PLASMA AND URINE OF PATIENTS TREATED WITH MESNA
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DOI：
10.1097/00007691-199504000-00009
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发表时间：
1995-04-01
影响因子：
2.5
通讯作者：
ALRAWITHI, S
中科院分区：
文献类型：
--
作者：
ELYAZIGI, A;YUSUF, A;ALRAWITHI, S
We describe in this report an expedient and accurate liquid chromatographic method for measurement of mesna and dimesna in plasma and urine. The separation of mesna and the internal standard (p-aminobenzoic acid, IS) was achieved on a 10-mu m, 8 mm (i.d.) x 10-cm C-18-Resolve cartridge in conjunction with radial compression system. An aqueous solution of sodium citrate (0.1 M), tetrabutyl ammonium phosphate (0.001 M), and triethylamine (1:10,000, vol/vol), adjusted to pH 5 with 85% phosphoric acid was used at a flow rate of 2 ml/min as a mobile phase. The compounds were detected in the effluent electrochemically at +450 mV. After an appropriate amount of IS was added, the plasma sample (100 mu l or fraction thereof) was deproteinized with an equal volume of 0.0825 M sulfuric acid containing sodium hexametaphosphate (1.25% wt/vol), whereas urine was diluted 1:50 with water and mixed 1:1 with an aqueous solution of sodium hexametaphosphate (1.25% wt/vol). Dimesna was reduced back to mesna with sodium borohydride before analysis of the total mesna. The peak height ratio (drug/IS) varied linearly with the concentration, and the correlation coefficient was >0.992 for both mesna and di mesna. The intrarun precision at different concentrations of mesna was equally good and the coefficient of variation was consistently
