Control of urea synthesis in fetal rat liver slices.
Control of urea synthesis in fetal rat liver slices.
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胎鼠肝切片中尿素合成的控制。
DOI：
10.1159/000241496
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
R. Vaillant
中科院分区：
文献类型：
--
作者：
A. Husson;A. Fairand;R. Vaillant
采用肝切片系统，以氯化铵为氮源，研究了胎鼠体内尿素的合成。在足月胎儿中，尿素的形成速率随着氯化铵浓度的增加而增加，达到100微米，并通过添加鸟氨酸而增强。尿素合成的发育模式与酶活性研究中发现的非常相似：尿素产量在胎儿时期略有增加，然后在出生时迅速上升。研究发现，胎儿肝脏对糖皮质激素有绝对的需求，以发展正常的尿素合成。讨论了肝脏匀浆中精氨酸琥珀酸合成酶活性测定的一致性。
Urea synthesis in fetal rats was studied using a liver slice system with ammonium chloride as nitrogen source. In term fetuses, the rate of urea formation increases with ammonium chloride concentration up to 100 microM and is enhanced by the addition of ornithine. The developmental pattern for urea synthesis is very similar to that found in the enzyme activity studies: urea production increases slightly during fetal life and then rises rapidly at birth. It is found that fetal liver has an absolute requirement for glucocorticosteroids to develop a normal urea synthesis. The accordance with argininosuccinate synthetase activity measured in liver homogenate is discussed.