Inaccuracies of nitric oxide measurement methods in biological media.
Inaccuracies of nitric oxide measurement methods in biological media.
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DOI：
10.1021/ac303787p
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发表时间：
2013-02-05
影响因子：
7.4
通讯作者：
Schoenfisch, Mark H.
中科院分区：
文献类型：
--
作者：
Hunter, Rebecca A.;Storm, Wesley L.;Coneski, Peter N.;Schoenfisch, Mark H.
尽管越来越多的报告的生物作用的一氧化氮（NO）作为一个功能的NO有效载荷，这种工作的有效性往往是值得怀疑的方式，其中NO的测量和/或溶液组成，其中NO是定量的。为了突出测量技术对于给定样品类型的重要性，从小分子NO供体（N-二醇二氮烯盐L-脯氨酸，PROLI/NO）和NO释放干凝胶膜产生的NO在许多生理缓冲液和流体、细胞培养基和细菌肉汤中使用Griess测定、化学发光分析仪和电流型NO传感器进行定量。尽管广泛使用，格里斯测定被证明是不准确的测量NO在许多媒体测试。相比之下，化学发光分析仪在大多数缓冲液中提供了极好的动力学信息，但对于蛋白质介质中的NO分析不切实际。电化学NO传感器使更大的灵活性，在各种媒体的空间分辨率的潜力，虽然在低于预期的NO总量与格里斯测定或化学发光。这项研究的结果突出了测量策略的重要性，准确的NO分析和报告NO为基础的生物活性。
Despite growing reports on the biological action of nitric oxide (NO) as a function of NO payload, the validity of such work is often questionable due to the manner in which NO is measured and/or the solution composition in which NO is quantified. To highlight the importance of measurement technique for a given sample type, NO produced from a small molecule NO donor (N-diazeniumdiolated l-proline, PROLI/NO) and a NO-releasing xerogel film were quantified in a number of physiological buffers and fluids, cell culture media, and bacterial broth using the Griess assay, a chemiluminescence analyzer, and an amperometric NO sensor. Despite widespread use, the Griess assay proved to be inaccurate for measuring NO in many of the media tested. In contrast, the chemiluminescence analyzer provided superb kinetic information in most buffers, but was impractical for NO analysis in proteinaceous media. The electrochemical NO sensor enabled greater flexibility across the various media with potential for spatial resolution, albeit at lower than expected NO totals versus either the Griess assay or chemiluminescence. The results of this study highlight the importance of measurement strategy for accurate NO analysis and reporting NO-based biological activity.
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DOI：
10.1016/j.niox.2009.07.002
发表时间：
2009-09
期刊：
Nitric oxide : biology and chemistry
影响因子：
--
作者：
Hall CN;Garthwaite J
通讯作者：
Garthwaite J
影响因子：
5
作者：
Guevara, I;Iwanejko, J;Szczudlik, A
通讯作者：
Szczudlik, A
影响因子：
7.4
作者：
Amatore, Christian;Arbault, Stephane;Koh, Alaric C. W.
通讯作者：
Koh, Alaric C. W.
影响因子：
3.2
作者：
Amatore, Christian;Arbault, Stephane;Koh, Alaric C. W.
通讯作者：
Koh, Alaric C. W.
影响因子：
--
作者：
Damodaran, Vinod B.;Joslin, Jessica M.;Reynolds, Melissa M.
通讯作者：
Reynolds, Melissa M.