A distinct population of Saccharomyces cerevisiae in New Zealand: evidence for local dispersal by insects and human-aided global dispersal in oak barrels
A distinct population of Saccharomyces cerevisiae in New Zealand: evidence for local dispersal by insects and human-aided global dispersal in oak barrels
复制标题
DOI：
10.1111/j.1462-2920.2009.02035.x
复制
发表时间：
2010-01-01
影响因子：
5.1
通讯作者：
Jun, Casey
中科院分区：
文献类型：
--
作者：
Goddard, Matthew R.;Anfang, Nicole;Jun, Casey
人类已经使用了S。酿酒厂制造酒精饮料至少有5000年的历史，现在这种超级模型研究生物体对我们生物学理解的进步至关重要。目前，S.酿酒酵母的研究表明，其种群包括不同的驯化和自然群体以及镶嵌株，但我们通常对塑造其种群结构的力量知之甚少。为了检验生态学和地理学在形成S.我们检测了172株酿酒酵母中的9个可变微卫星位点。酿酒酵母分离自两个自发的葡萄汁发酵，土壤，花，养蜂场和树皮在新西兰。贝叶斯分析表明，S。新西兰的酿酒酵母包括一个细分但杂交的群体，其异交率接近20%。一些菌株有助于自发发酵集群与新西兰土壤/树皮分离物，但其他集群与法国橡木桶分离物。似乎有些菌株已经被人类在橡木桶中传播到全球，而有些菌株则被昆虫传播到当地。这些数据表明，在形成S。酿酒厂的人口结构。
P>Humans have used S. cerevisiae to make alcoholic beverages for at least 5000 years and now this super-model research organism is central to advances in our biological understanding. Current models for S. cerevisiae suggest that its population comprises distinct domesticated and natural groups as well as mosaic strains, but we generally know little of the forces which shape its population structure. In order to test the roles that ecology and geography play in shaping the S. cerevisiae species we examined nine variable microsatellite loci in 172 strains of S. cerevisiae isolated from two spontaneous grape juice ferments, soil, flowers, apiaries and bark in New Zealand. Bayesian analysis shows that the S. cerevisiae in NZ comprise a subdivided but interbreeding population that out-crosses similar to 20% of the time. Some strains contributing to spontaneous ferments cluster with NZ soil/bark isolates, but others cluster with isolates from French oak barrels. It seems some strains have been globally dispersed by humans in oak barrels while some are locally vectored by insects. These data suggest geography is more important than ecology in shaping S. cerevisiae's population structure.