LOCALIZATION OF METHANOL DEHYDROGENASE IN 2 STRAINS OF METHYLOTROPHIC BACTERIA DETECTED BY IMMUNOGOLD LABELING
LOCALIZATION OF METHANOL DEHYDROGENASE IN 2 STRAINS OF METHYLOTROPHIC BACTERIA DETECTED BY IMMUNOGOLD LABELING
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DOI：
10.1128/aem.58.7.2302-2307.1992
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发表时间：
1992-07-01
影响因子：
4.4
通讯作者：
REMSEN, CC
中科院分区：
文献类型：
--
作者：
FASSEL, TA;BUCHHOLZ, LA;REMSEN, CC
制备了从甲基杆菌属菌株AM 1和甲基单胞菌属菌株A4纯化的甲醇脱氢酶的抗体。抗体制备物用于间接免疫金标记研究。用这种方法，发现甲醇脱氢酶优先定位于甲基营养型甲基杆菌属菌株AM 1的周质区域和甲烷营养型甲基单胞菌属菌株A4的胞质膜内。检测到抗体与其他甲基营养菌的交叉反应性。
Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph Methylobacterium sp. strain AM1 and to the intracytoplasmic membrane of the methanotroph Methylomonas sp. strain A4. Antibody cross-reactivity to other methylotrophic bacteria was detected.