Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics
Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics
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DOI：
10.1105/tpc.105.031542
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发表时间：
2005-08-01
期刊：
影响因子：
11.6
通讯作者：
Turner, SR
中科院分区：
文献类型：
--
作者：
Brown, DM;Zeef, LAH;Turner, SR
正向遗传筛选已经导致了参与次生细胞壁形成的几个基因的分离。然而，各种证据表明，所确定的基因清单并不详尽。为了解决这个问题，已经从经历次生细胞壁形成的组织中生成了微阵列数据，并用于鉴定与次生细胞壁特异性纤维素合成酶基因IRREGULAR XYLEM 1（IRX 1）和IRX 3表现出相似表达模式的基因。交叉引用该分析与公开可用的微阵列数据导致选择16个基因进行反向遗传分析。含有其中七个基因插入的系表现出明显的次生细胞壁缺陷特征的irx表型。只有一个线，含有插入的COBRA基因家族的成员，表现出纤维素含量大幅下降。被鉴定为次生细胞壁生物合成所必需的基因中有五个以前没有被鉴定过。这些基因可能定义了次生细胞壁形成的全新过程，并说明了将表达数据与反向遗传学相结合以解决基因功能的成功。
Forward genetic screens have led to the isolation of several genes involved in secondary cell wall formation. A variety of evidence, however, suggests that the list of genes identified is not exhaustive. To address this problem, microarray data have been generated from tissue undergoing secondary cell wall formation and used to identify genes that exhibit a similar expression pattern to the secondary cell wall - specific cellulose synthase genes IRREGULAR XYLEM1 ( IRX1) and IRX3. Cross-referencing this analysis with publicly available microarray data resulted in the selection of 16 genes for reverse genetic analysis. Lines containing an insertion in seven of these genes exhibited a clear irx phenotype characteristic of a secondary cell wall defect. Only one line, containing an insertion in a member of the COBRA gene family, exhibited a large decrease in cellulose content. Five of the genes identified as being essential for secondary cell wall biosynthesis have not been previously characterized. These genes are likely to define entirely novel processes in secondary cell wall formation and illustrate the success of combining expression data with reverse genetics to address gene function.