Noninvasive optical tracking of red fluorescent protein-expressing cancer cells in a model of metastatic breast cancer
Noninvasive optical tracking of red fluorescent protein-expressing cancer cells in a model of metastatic breast cancer
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DOI：
10.1593/neo.06304
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发表时间：
2006-10-01
期刊：
影响因子：
4.8
通讯作者：
Raman, Venu
中科院分区：
文献类型：
--
作者：
Winnard, Paul T., Jr.;Kluth, Jessica B.;Raman, Venu
我们评估了 Xenogen IVIS 200 成像系统在活体动物转移进展中基于实时荧光蛋白的光学成像的使用情况。我们发现在小鼠尸体模型实验中检测不到表达绿色荧光蛋白的细胞（100 x 10(6)）。然而，很容易检测到表达 tdTomato 的细胞数量减少了 10 倍。产生稳定的 MDA-MB-231-tdTomato 细胞的乳腺脂肪垫异种移植物，用于转移进展的成像。注射后两周，在注射部位很容易检测到几乎无法触及的肿瘤负荷。第 8 周时，对侧乳腺脂肪垫的小转移进行了成像，到第 13 周时，可检测到淋巴结转移。 15 周时，在胸腔区域内可检测到具有结节成分的转移瘤。 3D 图像重建表明荧光检测范围延伸至表面以下约 1 厘米。强烈的 tdTomato 荧光、≥ 620 nm 的成像（自发荧光最小化）以及 Xenogen 成像仪的灵敏度相结合，使这一切成为可能。本研究证明了使用 >= 620 nm 检测波长的内源表达荧光蛋白报告基因在转移进展过程中对癌细胞进行无创光学追踪的实用性。
We have evaluated the use of the Xenogen IVIS 200 imaging system for real-time fluorescence protein based optical imaging of metastatic progression in live animals. We found that green fluorescent protein expressing cells ( 100 x 10(6)) were not detectable in a mouse cadaver phantom experiment. However, a 10-fold lower number of tdTomato-expressing cells were easily detected. Mammary fat pad xenografts of stable MDA-MB-231-tdTomato cells were generated for the imaging of metastatic progression. At 2 weeks postinjection, barely palpable tumor burdens were easily detected at the sites of injection. At 8 weeks, a small contralateral mammary fat pad metastasis was imaged and, by 13 weeks, metastases to lymph nodes were detectable. Metastases with nodular composition were detectable within the rib cage region at 15 weeks. 3-D image reconstructions indicated that the detection of fluorescence extended to approximately 1 cm below the surface. A combination of intense tdTomato fluorescence, imaging at >= 620 nm ( where autofluorescence is minimized), and the sensitivity of the Xenogen imager made this possible. This study demonstrates the utility of the noninvasive optical tracking of cancer cells during metastatic progression with endogenously expressed fluorescence protein reporters using detection wavelengths of >= 620 nm.