A gene-driven recovery mechanism: Drosophila larvae increase feeding activity for post-stress weight recover.
A gene-driven recovery mechanism: Drosophila larvae increase feeding activity for post-stress weight recover.
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基因驱动的恢复机制：果蝇幼虫增加摄食活动以恢复应激后的体重。
DOI：
10.1002/arch.21440
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Y.
中科院分区：
文献类型：
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作者：
Ryuda;M.;Tabuchim M.;Matsumoto;M.;Matsumura;T.;Ochiai;M.;Hayakawa;Y.
应激后从体重减轻中恢复对所有生物体都很重要，尽管恢复机制还不完全清楚。我们正在努力澄清这些机制。在这里，我们记录了在35℃热应激1h后2-4h内黑腹果蝇幼虫的取食活动增强。在后应激期间，甜味味觉受体基因(Gr5a、Gr43a、Gr64a和Gr64f)的表达水平上升，而苦味味觉受体基因Gr66a和Gr33a的表达水平下降，而非典型味觉受体Gr、Gr68a的表达没有变化。4℃冷应激后，幼虫口部原肌球蛋白和三磷酸腺苷合成酶亚基的表达水平在3~5h明显增加，Gr5a表达上调，Gr66a下调。我们推断，应激后幼虫摄食活动和体重恢复的上调是通过增加口部肌肉运动的能力和味觉生理变化来调节的。我们认为，果蝇和可能的昆虫一般都表达了一种有效的机制，可以在后应激时期从体重减轻中恢复过来。
Recovery from weight loss after stress is important for all organisms, although the recovery mechanisms are not fully understood. We are working to clarify these mechanisms. Here, we recorded enhanced feeding activity ofDrosophila melanogasterlarvae from 2 to 4 h after heat stress at 35°C for 1 h. During the post‐stress period, expression levels of sweet taste gustatory receptor genes (Grs),Gr5a,Gr43a,Gr64a, andGr64f, were elevated, whereas bitter tasteGrs,Gr66a, andGr33a, were decreased in expression and expression of a non‐typical taste receptorGr,Gr68a, was unchanged. Similar upregulation ofGr5aand downregulation ofGr66awas recorded after cold stress at 4°C. Expression levels of tropomyosin and ATP synthase ß subunit were significantly increased in larval mouth parts around 3 to 5 h after the heat stress. We infer that up‐regulation of post‐stress larval feeding activity, and weight recovery, is mediated by increasing capacity for mouth part muscular movements and changes in taste sensing physiology. We propose thatDrosophilalarvae, and likely insects generally, express an efficient mechanism to recover from weight loss during post‐stress periods.