Nonsense-mediated decay mutants do not affect programmed-1 frameshifting
Nonsense-mediated decay mutants do not affect programmed-1 frameshifting
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DOI：
10.1017/s1355838200000443
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发表时间：
2000-07-01
期刊：
影响因子：
4.5
通讯作者：
Farabaugh, PJ
中科院分区：
文献类型：
--
作者：
Bidou, L;Stahl, G;Farabaugh, PJ
某些mRNA中的序列对核糖体进行编程，使其经历非经典翻译事件、翻译移码、翻译跳跃或终止通读。这些序列被称为重新编码位点，因为它们导致核糖体暂时改变其编码规则。顺式和反式作用因子敏感地调节重编码事件的效率。在尝试定量这些因素的影响，我们已经开发了一个双报告载体，使用lacZ和luc基因直接测量重编码效率。我们能够确认在各种位点调节移码或通读效率的几个因素的影响。令人惊讶的是，我们无法证实被称为监视复合物的因子复合物调节翻译移码。该复合物调节含有无义密码子的mRNA的降解，并且我们证实它也影响无义抑制的效率。我们的数据表明，监视复合物不是翻译准确性的一般调节器，但其作用与翻译终止和起始过程密切相关。
Sequences in certain mRNAs program the ribosome to undergo a noncanonical translation event, translational frameshifting, translational hopping, or termination readthrough. These sequences are termed recoding sites, because they cause the ribosome to change temporarily its coding rules. Cis and trans-acting factors sensitively modulate the efficiency of recoding events. In an attempt to quantitate the effect of these factors we have developed a dual-reporter vector using the lacZ and luc genes to directly measure recoding efficiency. We were able to confirm the effect of several factors that modulate frameshift or readthrough efficiency at a variety of sites. Surprisingly, we were not able to confirm that the complex of factors termed the surveillance complex regulates translational frameshifting. This complex regulates degradation of nonsense codon-containing mRNAs and we confirm that it also affects the efficiency of nonsense suppression. Our data suggest that the surveillance complex is not a general regulator of translational accuracy, but that its role is closely tied to the translational termination and initiation processes.