Triggered Release of Bacteriophage K from Agarose/Hyaluronan Hydrogel Matrixes by Staphylococcus aureus Virulence Factors
Triggered Release of Bacteriophage K from Agarose/Hyaluronan Hydrogel Matrixes by Staphylococcus aureus Virulence Factors
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DOI：
10.1021/cm503974g
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发表时间：
2014-12-23
影响因子：
8.6
通讯作者：
Jenkins, A. Toby A.
中科院分区：
文献类型：
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作者：
Bean, Jessica E.;Alves, Diana R.;Jenkins, A. Toby A.
近年来，水凝胶作为安全、生物相容的伤口愈合材料已被广泛使用。在这里，我们研究了使用复合水凝胶赋予抗菌水凝胶系统的触发机制。该系统由双层水凝胶结构组成：含有抗微生物病毒噬菌体K（FK）的下层琼脂糖层和由产生水凝胶触发剂的光交联透明质酸甲基丙烯酸酯（HAMA）形成的上层。该触发物对透明质酸酶敏感，透明质酸酶是已知由大多数金黄色葡萄球菌菌株分泌的酶。在透明质酸酶的存在下，HAMA被降解，将FK释放到周围环境中，从而继续杀死周围的细菌。我们的结果表明，在与透明质酸酶（纯化的或从S。金黄色葡萄球菌），随着降解的进行，在HAMA中形成大孔，这促进FK释放。
The use of hydrogels as safe, biocompatible materials for wound healing has been widely utilized in recent years. Here, we investigated the use of a composite hydrogel to impart a trigger mechanism into an antimicrobial hydrogel system. The system was comprised of a bilayer hydrogel architecture: a lower agarose layer containing the antimicrobial virus Bacteriophage K (FK) and an upper layer formed of photo-cross-linkable hyaluronic acid methacrylate (HAMA) which creates the hydrogel trigger. This trigger is sensitive to the enzyme hyaluronidase, an enzyme known to be secreted by the majority of Staphylococcus aureus strains. In the presence of hyaluronidase, HAMA is degraded, releasing FK into the surrounding environment which consequently go on to kill surrounding bacteria. Our results show that on incubation with hyaluronidase (purified or from S. aureus), large pores form in HAMA as degradation goes on, which facilitates FK release.