Integrating DNA Sequences with Morphological Analysis Clarifies Phylogenetic Position ofSalvia grandifolia(Lamiaceae): An Enigmatic Species Endemic to Southwestern China
Integrating DNA Sequences with Morphological Analysis Clarifies Phylogenetic Position ofSalvia grandifolia(Lamiaceae): An Enigmatic Species Endemic to Southwestern China
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DOI：
10.1086/709134
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发表时间：
2020-10-01
影响因子：
2.3
通讯作者：
Xiang, Chun-Lei
中科院分区：
文献类型：
--
作者：
Hu, Guo-Xiong;Liu, En-De;Xiang, Chun-Lei
研究前提。大叶鼠尾草是一种仅产于中国西南部横断山脉的狭义特有物种。先前的研究表明东亚丹参属形成一个分支Salviasubg.Glutinaria，并提出S. grandifolia，根据地理分布，应包括在 Glutinaria 中。然而，花萼和雄蕊特征表明S. grandifolia 可能与 subg.Sclarea、subg.Salvia 和非正式 subg. 密切相关。 “异相”，主要发现于中亚和西南亚、欧洲和地中海地区。自从 S. grandifoliais是一种稀有物种，从未被纳入任何分子系统发育研究，该物种的系统发育地位仍不确定。方法论。 S的系统发育位置。 grandifolia 是利用 DNA 序列以及形态学和细胞学证据推断的。我们使用两个核核糖体 DNA 区域（内部转录间隔区和外部转录间隔区）和一种叶绿体标记 (rpl32-trnL)，并结合最大似然法和贝叶斯推理方法来检查系统发育关系。关键结果。 S内体细胞的染色体数目。大叶为 2n=4x=40。 S. 并没有像预期那样与东亚亚群谷类 (Glutinaria) 归为一类。在 nrDNA 和 cpDNA 系统发育分析中，grandifolia 被嵌入 subg.Sclarea 中。结论。系统发育和形态学证据表明S。 grandifoliais 是 Sclarea 亚属的成员，因此东亚鼠尾草在地理意义上是非单系的。表面上看，丹参从西南亚传播到东亚至少发生过两次。
Premise of research. Salvia grandifoliais a narrowly endemic species known only from the Hengduan Mountains of southwestern China. Previous studies have shown that East AsianSalviaspecies form a clade,Salviasubg.Glutinaria, and suggested thatS. grandifolia, on the basis of geographical distribution, should be included withinGlutinaria. Calyx and staminal features, however, indicate thatS. grandifoliamay be closely related to taxa of subg.Sclarea, subg.Salvia, and the informal subg. "Heterosphace," which are primarily found in central and southwest Asia, Europe, and the Mediterranean region. SinceS. grandifoliais a rare species and has never been included in any molecular phylogenetic studies, the phylogenetic position of this species remains uncertain. Methodology. The phylogenetic position ofS. grandifoliawas inferred using DNA sequences as well as morphological and cytological evidence. We used two nuclear ribosomal DNA regions (internal transcribed spacers and external transcribed spacers) and one chloroplast marker (rpl32-trnL) in concert with maximum likelihood and Bayesian inference approaches to examine phylogenetic relationships. Pivotal results. The chromosome number of somatic cells withinS. grandifoliawas 2n=4x=40. Rather than being grouped with taxa of the East Asian subg.Glutinariaas expected,S. grandifoliawas embedded within subg.Sclareain both nrDNA and cpDNA phylogenetic analyses. Conclusions. Phylogenetic and morphological evidence indicates thatS. grandifoliais a member of subg.Sclarea, and thus East AsianSalviais nonmonophyletic in a geographic sense. Dispersal ofSalviafrom southwest Asia to East Asia has ostensibly occurred at least twice.