[Protective effect of safflower yellow injection against rat MIRI by TLR-NF-kappaB inflammatory pathway].
[Protective effect of safflower yellow injection against rat MIRI by TLR-NF-kappaB inflammatory pathway].
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DOI：
10.19540/j.cnki.cjcmm.20190201.001
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发表时间：
2019-06-01
期刊：
影响因子：
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通讯作者：
Liu, Jian-Xun
中科院分区：
文献类型：
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作者：
Li, Ling-Mei;Fu, Jian-Hua;Liu, Jian-Xun
本研究旨在探讨红花黄注射液对大鼠心肌缺血再灌注损伤（MIRI）炎症反应的调节机制。将雄性Wistar大鼠随机分为假手术组、模型组、合北双组和红花黄注射液高、中、低剂量组。结扎左冠状动脉前降支建立MIRI模型。HE染色观察心肌组织病理学改变；TTC染色检测心肌梗死面积；采用生化法或酶联免疫吸附法（ELISA）检测肿瘤坏死因子- α (tnf - α)、白细胞介素-6（IL-6）、血清肌酸激酶（CK）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）的含量及变化。Western blot法检测心肌组织中toll样受体4（TLR4）和核因子- κ b （nf - κ b p65）蛋白的表达。结果显示，与假手术组比较，模型组大鼠心肌排列紊乱，间质严重水肿，心肌梗死面积明显增大，血清AST、CK、LDH活性升高，tnf - α、IL-6含量显著升高；心肌组织中TLR4和NF-kappaB（p65）蛋白的表达水平也升高。与模型组比较，鹤北双组和红花黄注射液高、中、低剂量组大鼠心肌组织排列整齐；水肿明显减轻；心肌梗死面积明显减小；血清AST、CK、LDH活性及tnf - α、IL-6水平显著降低，心肌组织TLR4、NF-kappaB（p65）蛋白表达水平降低。与河北双组比较，红花黄注射液高、中、低剂量组心肌梗死面积均较大；血清中AST、CK、LDH活性及tnf - α、IL-6含量升高，组织中TLR4、NF-kappaB（p65）蛋白表达水平差异无统计学意义。提示红花黄注射液具有明显的抗miri作用，其机制可能与调节TLR-NF-kappaB通路抑制炎症反应有关。
This study was to investigate the mechanism of safflower yellow injection for regulating inflammatory response against myocardial ischemia-reperfusion injury( MIRI) in rats. Male Wistar rats were randomly divided into sham operation group,model group,Hebeishuang group,safflower yellow injection high,medium and low dose groups. MIRI model was established by ligating left anterior descending coronary artery. Myocardial histopathological changes were observed by HE staining; myocardial infarct size was detected by TTC staining; content and changes of tumor necrosis factor-alpha( TNF-alpha) and interleukin-6( IL-6),serum creatine kinase( CK),aspartate aminotransferase( AST),and lactate dehydrogenase( LDH) were detected by biochemical method or enzyme-linked immunosorbent assay( ELISA). Western blot assay was used to detect the protein expression of Toll-like receptor 4( TLR4) and nuclear factor-kappaB( NF-kappaB p65) in myocardial tissues. The results showed that as compared with the sham operation group,the myocardial arrangement of the model group was disordered,with severe edemain the interstitial,significantly increased area of myocardial infarction,increased activities of AST,CK and LDH in serum,and significantly increased contents of TNF-alpha and IL-6; the expression levels of TLR4 and NF-kappaB( p65) protein in myocardial tissues were also increased. As compared with the model group,the myocardial tissues were arranged neatlyin the Hebeishuang group and safflower yellow injection high,medium and low dose groups; the edema was significantly reduced; the myocardial infarct size was significantly reduced; the serum AST,CK,LDH activity and TNF-alpha,IL-6 levels were significantly decreased,and the expression levels of TLR4 and NF-kappaB( p65) protein in myocardial tissues were decreased. As compared with the Hebeishuang group,the myocardial infarct size was larger in the safflower yellow injection high,medium and low dose groups; the activities of AST,CK and LDH in serum and the contents of TNF-alpha and IL-6 in serum were higher,but there was no statistically significant difference in the expression levels of TLR4 and NF-kappaB( p65) protein in tissues. It is suggested that safflower yellow injection has a significant anti-MIRI effect,and its mechanism may be related to the regulation of TLR-NF-kappaB pathway to inhibit inflammatory response.