Multi-analyte SPR immunoassays for environmental biosensing of pesticides
Multi-analyte SPR immunoassays for environmental biosensing of pesticides
复制标题
DOI：
10.1007/s00216-006-0800-z
复制
发表时间：
2007-02-01
影响因子：
4.3
通讯作者：
Lechuga, L. M.
中科院分区：
文献类型：
--
作者：
Mauriz, E.;Calle, A.;Lechuga, L. M.
采用双通道表面等离子体共振（SPR）生物传感器对环境相关农药进行多分析物检测。SPR仪器的特殊设计允许通过不同的固定形式测定几种分析物（滴滴涕、毒死蜱和西威因）。首先，通过在SPR系统的每个通道上分别流动毒死蜱、西威因或滴滴涕样品，其中相应的识别元件先前已固定，可以同时进行农药监测。第二种方法是将多个分析物识别元件组合固定在单个流动池的传感表面上。在这种格式中，所有三种农药的分析时间根据再生循环的次数从40到60分钟不等。在大多数情况下，无论分析格式如何，目标分析物的检测限都达到了相似的检测限，灵敏度值为纳克/升水平（18-50 ng L-1）。通过重复使用相同的传感器表面超过200个分析周期，证明了分析的重复性，而没有生物传感器对非相关分析物的响应表明了分析的特异性和可靠性。SPR仪器，包括光学、电子和微流体，已经由sena公司商业化。
Multi-analyte detection of environmentally relevant pesticides is performed by using a two-channelled surface plasmon resonance (SPR) biosensor. The special design of the SPR instrument allows the determination of several analytes (DDT, chlorpyrifos and carbaryl) via different immobilization formats. First, simultaneous pesticide monitoring is possible by flowing chlorpyrifos, carbaryl or DDT samples separately over each channel of the SPR system, wherein their corresponding recognition element was previously immobilized. The second approach is based on the multiple and combined immobilization of several analyte recognition elements on the sensing surface of one individual flow cell. In this format, the analysis time for all three pesticides varied from 40 to 60 min depending on the number of regeneration cycles. In most cases, similar detection limits were attained for the target analyte irrespective of the assay format, with sensitivity values at the nanogram per litre level (18-50 ng L-1). The assay reproducibility was proved through the repeated use of the same sensor surface for over more than 200 assay cycles, whereas the absence of biosensor response to non-related analytes showed the specificity and reliability of the analysis. The SPR instrument, including optics, electronics and microfluidics, is already commercialised by the company SENSIA, SL.