Expression characteristics of astrocyte elevated gene-1 (AEG-1) in tongue carcinoma and its correlation with poor prognosis
Expression characteristics of astrocyte elevated gene-1 (AEG-1) in tongue carcinoma and its correlation with poor prognosis
复制标题
舌癌中星形胶质细胞升高基因1(AEG-1)的表达特征及其与不良预后的相关性
DOI：
10.1016/j.canep.2012.10.007
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发表时间：
2013-04-01
影响因子：
2.6
通讯作者：
Zhou, Wenwen
中科院分区：
文献类型：
--
作者：
Ke, Zun-fu;He, Shanyang;Zhou, Wenwen
星形胶质细胞升高基因-1 （AEG-1）在多种人类癌症中表达增加，在肿瘤发生和发展中起重要作用。本研究旨在探讨AEG-1在舌鳞癌（TSCC）中的临床病理特征及预后意义。采用免疫组化（IHC）方法检测93例TSCC石蜡包埋组织中AEG-1蛋白的表达。采用Real-time PCR和western blot方法检测同一患者4对原发TSCC及癌旁非癌组织中AEG-1的表达。免疫组化结果显示，TSCC组织中AEG-1的阳性率（48.39%,45/93）高于正常舌组织（10.00%,3/30）（P < 0.001）。Western blot和RT-PCR进一步证实了TSCC组织与匹配的邻近非癌组织之间的差异。同时，AEG-1蛋白水平与分化程度（P < 0.001）、临床分期（P < 0.001）、T分型（P = 0.007）、N分型（P = 0.012）呈正相关。此外，AEG-1表达较高的患者总生存时间较短。多因素分析（Cox回归）也提示AEG-1表达是TSCC的独立预后指标（P = 0.043）。我们的研究结果表明，AEG-1的表达与TSCC的癌变和进展密切相关，可能是TSCC患者预后评估的一种新的有价值的预测因子。(C) 2012 Elsevier Ltd.版权所有。
Astrocyte elevated gene-1 (AEG-1) expression is increased in diverse human cancers and plays a vital role in tumorigenesis and progression. The aim of this study was to investigate the clinicopathologic features and prognostic significance of AEG-1 in squamous cell carcinoma of the tongue (TSCC). Immunohistochemistry (IHC) was performed to examine AEG-1 protein expression in paraffin-embedded tissues from 93 patients with TSCC. Real-time PCR and western blot analyses were employed to examine AEG-1 expression in 4 pairs of primary TSCC and adjacent non-cancerous tissues from the same patient. Immunohistochemical results revealed that the positive rate for AEG-1 in TSCC tissues (48.39%, 45/93) was higher than that in the normal tongue tissues (10.00%, 3/30) (P < 0.001). These results were further confirmed between TSCC tissues and matched adjacent non-cancerous tissues by Western blot and RT-PCR. Simultaneously, AEG-1 protein level was positively correlated with differentiation degree (P < 0.001), clinical stage (P < 0.001), T classification (P = 0.007) and N classification (P = 0.012). Furthermore, patients with higher AEG-1 expression had shorter overall survival time. Multivariate analysis (Cox regression) also suggested that AEG-1 expression was an independent prognostic indicator for TSCC (P = 0.043). Our results indicate that AEG-1 expression is closely associated with carcinogenesis and progression of TSCC, and may represent a novel and valuable predictor for prognostic evaluation of TSCC patients. (C) 2012 Elsevier Ltd. All rights reserved.