Intra‐oral detection of potent odorants using a modified stir‐bar sorptive extraction system in combination with HRGC–O, known as the buccal odour screening system (BOSS)
Intra‐oral detection of potent odorants using a modified stir‐bar sorptive extraction system in combination with HRGC–O, known as the buccal odour screening system (BOSS)
复制标题
使用改良的搅拌棒吸附萃取系统与 HRGC-O 相结合，进行口腔内强效气味检测，称为口腔气味筛查系统 (BOSS)
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
F. Welle
中科院分区：
文献类型：
--
作者：
A. Buettner;F. Welle
这项工作的主题是开发一种新的方法，可用于识别口腔内的强效气味剂，并确定它们在口腔内的停留时间。该技术，称为口腔气味筛选系统（BOSS），是基于改进的搅拌棒吸附萃取（SBSE）系统。它包括在优化的体内采样条件下，在进食后的规定时间内对气味化合物进行口腔内提取，并通过高分辨率气相色谱-嗅觉测定法（HRGC-O）进行分析。所获得的分析数据与时间分辨鼻后香气评价有关。这允许表征与体内香气浓度变化相关的口服香气应用后引起的延长的香气感知。为了验证该系统，在水溶液中对重要的芳香化合物进行模型测定，以确保在给定的提取条件下所应用的SBSE系统具有足够的灵敏度。
The topic of this work was to develop a novel approach that can be used to identify potent odorants intraorally and to determine their dwell time within the oral cavity. The technique, called buccal odour screening system (BOSS), is based on a modified stir bar sorptive extraction (SBSE) system. It comprises the intra-oral extraction of odour compounds at defined times after food consumption under optimized in vivo sampling conditions, together with analysis via high resolution gas chromatography-olfactometry (HRGC-O). The obtained analytical data were related to time-resolved retronasal aroma evaluation. This allowed the characterization of prolongued aroma perception elicited after oral aroma application in relation to aroma concentration changes in vivo. For validation of the system, model assays were performed on important aroma compounds in aqueous solutions to ensure sufficient sensitivity of the applied SBSE system under the given extraction conditions.