Application of fluorescence correlation spectroscopy to the measurement of local temperature in solutions under optical trapping condition
Application of fluorescence correlation spectroscopy to the measurement of local temperature in solutions under optical trapping condition
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DOI：
10.1021/jp065156l
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发表时间：
2007-03-08
影响因子：
3.3
通讯作者：
Miyasaka, Hiroshi
中科院分区：
文献类型：
--
作者：
Ito, Syoji;Sugiyama, Takashi;Miyasaka, Hiroshi
荧光相关光谱 (FCS) 用于在 1064 nm 近红外 (NIR) 激光束存在的激光捕获条件下定量评估溶液中小于 1 μm 的小区域的局部加热。根据 FCS 获得的荧光分子的平移扩散系数，在乙二醇、乙醇和水中，温升与入射近红外激光功率 Delta T/Delta P 之间的关系分别确定为 62 +/- 6、49 +/- 7 和 23 +/- 1 deg K/W，而在氘化水中没有观察到明显的温度升高。 Delta T/Delta P 的值作为 alpha/lambda 的函数线性增加（alpha 是溶剂在该波长下的消光系数，lambda 是介质的导热系数）。通过将本方法的结果与以往其他各种方法的结果进行比较，讨论了本方法测量局部温升的有效性和适用性。
Fluorescence correlation spectroscopy (FCS) was applied to the quantitative evaluation of the local heating in small domains < 1 mu m in solutions under the laser trapping condition in the presence of a near-infrared (NIR) laser beam at 1064 nm. On the basis of the translational diffusion coefficient of fluorescent molecules obtained by FCS, the relationship between temperature rise and the incident NIR laser power, Delta T/Delta P, were determined to be 62 +/- 6, 49 +/- 7, and 23 +/- 1 deg K/W in ethylene glycol, ethanol, and water, respectively, while no remarkable temperature increase was observed for deuterated water. The value of Delta T/Delta P linearly increased as a function of alpha/lambda (alpha is the extinction coefficient of solvent at the wavelength and lambda is the thermal conductivity of the medium). The validity and the applicability of the present method for the measurement of the local temperature increase were discussed by comparing the present results with previous ones by other various methods.