Effects of alkaloids from Sophora flavescens on osteoblasts infected with Staphylococcus aureus and osteoclasts
Effects of alkaloids from Sophora flavescens on osteoblasts infected with Staphylococcus aureus and osteoclasts
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DOI：
10.1002/ptr.6069
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发表时间：
2018-07-01
影响因子：
7.2
通讯作者：
Shou, Dan
中科院分区：
文献类型：
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作者：
Wang, Xuping;Zheng, Rongzong;Shou, Dan
慢性骨髓炎主要是由金黄色葡萄球菌（S.aureus）感染引起的。抗生素是常用的;然而，这是一个挑战，以促进骨愈合。本研究的目的是研究苦参生物碱（ASF）对金黄色葡萄球菌感染的大鼠颅骨成骨细胞（ROBs）和健康破骨细胞的体外作用。细胞增殖和碱性磷酸酶，白细胞介素-6，和肿瘤坏死因子的活性进行了测定感染的ROBs;抗酒石酸酸性磷酸酶通过酶联免疫吸附试验在破骨细胞进行了评价。通过逆转录-聚合酶链反应和蛋白质印迹分析，骨形态发生蛋白2，runt相关转录因子2，骨保护素和核因子κ B受体激活剂配体的mRNA和蛋白质表达水平进行了评估，分别在感染ROBs。结果表明，ASF通过调节骨形态发生蛋白2、Runt相关转录因子、骨保护素和核因子κ B受体激活因子配体mRNA和蛋白表达水平，增加了未感染ROBs和万古霉素处理的感染ROBs的活力。此外，炎症因子肿瘤坏死因子的分泌减少，碱性磷酸酶活性增加，抑制破骨细胞的活力和抗酒石酸酸性磷酸酶活性。因此，草药ASF有潜力作为一种新的治疗慢性骨髓炎。
Chronic osteomyelitis is primarily caused by infection with Staphylococcus aureus (S.aureus). Antibiotics are commonly administered; however, it is a challenge to promote bone healing. The aim of this study was to investigate the in vitro effects of alkaloids from the herbal remedy Sophora flavescens (ASF) on rat calvarial osteoblasts (ROBs) infected with S.aureus and healthy osteoclasts. Cell proliferation and alkaline phosphatase, interleukin-6, and tumour necrosis factor- activity was measured in infected ROBs; tartrate-resistant acid phosphatase was evaluated in osteoclasts via enzyme-linked immunosorbent assay. The mRNA and protein expression levels of bone morphogenetic protein 2, runt-related transcription factor 2, osteoprotegerin, and receptor activator of nuclear factor kappa-B ligand were assessed in infected ROBs through reverse transcription-polymerase chain reaction and western blotting analysis, respectively. Results indicated that ASF increased the viability of uninfected ROBs and infected ROBs treated with vancomycin via regulation of bone morphogenetic protein 2, runt-related transcription factor, osteoprotegerin, and receptor activator of nuclear factor kappa-B ligand mRNA and protein expression levels. In addition, the secretion of the inflammatory factor tumour necrosis factor- was decreased and alkaline phosphatase activity was increased, inhibiting the viability of osteoclasts and tartrate-resistant acid phosphatase activity. Therefore, the herbal remedy ASF has potential as a new treatment for chronic osteomyelitis.