Effect of experimentally induced calcium deficiency on the developmental expression of collagen types in chick embryonic skeleton.
Effect of experimentally induced calcium deficiency on the developmental expression of collagen types in chick embryonic skeleton.
复制标题
实验诱导的缺钙对鸡胚胎骨骼中胶原蛋白类型发育表达的影响。
DOI：
10.1016/0012-1606(83)90232-4
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Lynch,MH
中科院分区：
文献类型：
--
作者：
Tuan,RS;Lynch,MH
为了研究胚胎缺钙对胚胎骨骼形成中细胞分化过程的影响，将鸡胚维持在体外长期无壳培养中。蛋壳通常在发育过程中为胚胎提供超过 120 毫克的钙，但由于蛋壳的缺失，导致骨骼形成严重迟缓和异常。通过检查正常和无壳胚胎的软骨内骨和膜内骨中胶原蛋白类型合成作为发育年龄的函数，评估发育过程中骨骼成分的细胞分化模式。从这些不同发育阶段的胚胎获得的骨骼组织在含有[3H]Pro的培养基中维持在短期器官培养物中。从这些组织中分离出代谢标记的胶原蛋白，并基于电泳、离子交换色谱、差异盐分级分离、区域沉淀色谱和 CNBr 肽图谱进行生化分型。结果表明，与时间上相当的正常对照相比，来自无壳胚胎的缺钙骨骼元件似乎无法成熟为完整的骨组织，而是表现出部分软骨表型，并表达软骨特异性 II 型胶原蛋白。
In order to investigate the effect of embryonic calcium deficiency on the cellular differentiation processes in embryonic skeletogenesis, chick embryos were maintained in long-term shell-less culturesin vitro. The absence of the eggshell, which normally provides over 120 mg of calcium to the embryo during the course of development, resulted in severely retarded and anomalous skeletal formation. The pattern of cytodifferentiation in the skeletal elements during development was assessed by examining collagen type synthesis in both endochondral and intramembranous bones of normal and shell-less embryos as a function of developmental age. Skeletal tissues obtained from these embryos at various developmental stages were maintained in short-term organ culture in medium containing [3H]Pro. The metabolically labeled collagen was isolated from these tissues and typed biochemically based on electrophoresis, ion-exchange chromatography, differential salt fractionation, zone precipitation chromatography, and CNBr peptide mapping. The results indicate that, compared to chronologically equivalent normal controls, calcium-deficient skeletal elements from shell-less embryos appeared to fail to mature into complete bony tissues and instead exhibited partial cartilage phenotype with the expression of cartilage-specific type II collagen.