Effects of pore size and implant volume of porous hydroxyapatite/collagen (HAp/Col) on bone formation in a rabbit bone defect model.
Effects of pore size and implant volume of porous hydroxyapatite/collagen (HAp/Col) on bone formation in a rabbit bone defect model.
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DOI：
10.11480/jmds.550111
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发表时间：
2008-03
影响因子：
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通讯作者：
A. Tsuchiya;S. Sotome;Y. Asou;M. Kikuchi;Y. Koyama;T. Ogawa;J. Tanaka;K. Shinomiya
中科院分区：
文献类型：
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作者：
A. Tsuchiya;S. Sotome;Y. Asou;M. Kikuchi;Y. Koyama;T. Ogawa;J. Tanaka;K. Shinomiya
制备了一种由羟基磷灰石纳米晶和胶原组成的多孔羟基磷灰石/胶原复合材料(HAP/Col)。本研究制备了不同孔径(160或290微米)的圆柱形(直径5 mm，高度3 mm)多孔HAP/Col种植体，并利用兔骨缺损模型评价了孔径大小和植入体积对植入效果的影响。种植体组植入1~3枚(直径5 mm，总高度9 mm)种植体，植入胫骨近端前内侧骨孔内，对照组未植入。组织学观察显示，在植入后两周，骨形成不仅从骨膜开始，而且在种植体与骨髓接壤的区域开始形成。四周后，骨形成从骨髓腔一侧扩展到种植体中心，尤其是那些毛孔较大的种植体。12周后，4组种植体出现皮质缺损修复和种植体吸收，这被认为是自然骨重建机制的结果。对照组骨膜发育成骨，骨髓腔内无骨诱导，4周后骨孔基本愈合。PQCT分析显示，大孔种植体的骨组织扩张率高于小孔种植体组。这些数据证明了多孔HAP/Col的骨传导性以及其多孔结构的重要性。
A porous hydroxyapatite/collagen composite (HAp/Col) was developed that consists of hydroxyapatite nanocrystals and atelocollagen. In this study, cylindrical (diameter: 5 mm, height: 3 mm) porous HAp/Col implants with different pore sizes (diameter: 160 or 290 microm) were prepared, and the influences of pore size and implanted volume were evaluated using a rabbit bone defect model. In the implant groups, one or three (diameter: 5 mm, total height: 9 mm) implants were transplanted into bone holes created in the anteromedial site of the proximal tibiae, while a group without implantation was used as a control. Histological observation revealed that at two weeks after implantation, bone formation was initiated not only from the periosteum but in regions where the implants bordered on bone marrow. At four weeks, bone formation expanded from the marrow cavity side into the center of the implants, particularly in those implants with large pores. At twelve weeks, four implant groups showed repair of cortical defects and implant absorption, which was thought to be the result of natural bone remodeling mechanisms. The control group showed bone formation developed from the periosteum without bone induction in the marrow cavity, and at four weeks, the bone hole was almost healed. pQCT analysis revealed that the expansion rates of bone tissue were higher in the large-pore implant groups than in the small-pore groups. These data demonstrate the osteoconductivity of porous HAp/Col and the importance of its porous structure.