Protective action of indapamide, a thiazide-like diuretic, on ischemia-induced injury and barrier dysfunction in mouse brain microvascular endothelial cells.
Protective action of indapamide, a thiazide-like diuretic, on ischemia-induced injury and barrier dysfunction in mouse brain microvascular endothelial cells.
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DOI：
10.1254/jphs.sc0060222
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发表时间：
2007
影响因子：
3.5
通讯作者：
T. Nishioku;F. Takata;A. Yamauchi;N. Sumi;I. Yamamoto;A. Fujino;M. Naito;T. Tsuruo;H. Shuto;Y. Kataoka
中科院分区：
文献类型：
--
作者：
T. Nishioku;F. Takata;A. Yamauchi;N. Sumi;I. Yamamoto;A. Fujino;M. Naito;T. Tsuruo;H. Shuto;Y. Kataoka
本研究的目的是阐明吲达帕胺对体外血脑屏障（BBB）缺血性损伤的影响。在此采用的缺血/再灌注条件显著降低了小鼠脑毛细血管内皮细胞（MBEC 4）的活力，吲达帕胺改善了这种效果。缺血增加了MBEC 4细胞对两种细胞转运标记物荧光素钠和伊文思蓝白蛋白的通透性。吲达帕胺可降低缺血诱导的细胞通透性增高。这些结果表明，吲达帕胺可能对缺血引起的损伤和功能障碍的血脑屏障的保护作用。
The aim of the present study was to elucidate the effects of indapamide on ischemic damage to the blood-brain barrier (BBB) in vitro. The ischemia/reperfusion conditions employed here significantly decreased the viability of mouse brain capillary endothelial (MBEC4) cells, an effect ameliorated by indapamide. Ischemia increased the permeability of MBEC4 cells to two cellular transport markers, sodium fluorescein and Evan's blue-albumin. Indapamide reduced the ischemia-induced hyperpermeability of cells. These results suggest that indapamide may have a protective role against ischemia-induced injury and dysfunction of the BBB.