Antibodies to Flaviviruses in Wild Ducks Captured in Hokkaido, Japan: Risk Assessment of Invasive Flaviviruses
Antibodies to Flaviviruses in Wild Ducks Captured in Hokkaido, Japan: Risk Assessment of Invasive Flaviviruses
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DOI：
10.1089/vbz.2008.0111
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发表时间：
2009-06-01
影响因子：
2.1
通讯作者：
Asakawa, Mitsuhiko
中科院分区：
文献类型：
--
作者：
Saito, Mika;Osa, Yuichi;Asakawa, Mitsuhiko
最近，日本脑炎病毒（JEV）和西尼罗河病毒（WNV）的分布已扩展到新的领域。西尼罗河病毒入侵日本引起了人们的高度关注。鸟类的迁徙可能与这些黄病毒的分布范围扩大有关。本研究于2005年和2006年秋季在日本乙型脑炎病毒低活动区北海道中部捕获野鸭92只，其中迁徙繁殖鸭20只，鸭嘴兽50只，冬候鸟16只，冬候鸟6只。采用90%和50%病灶减少中和试验（FRNT 90和FRNT 50）对黄病毒感染进行血清流行病学分析。在92份血清样本中，在FRNT90中分别有1份（1.1%）和5份（5.4%）WNV特异性和JEV特异性抗体检测阳性，在FRNT50中分别有61份（66.3%）和79份（85.9%）WNV和JEV检测阳性。这些结果表明，本研究中的野鸭已经暴露于黄病毒。这些结果以及黄病毒的公认分布和个别鸭种的迁徙路线强烈表明，本研究中捕获的鸟类在飞行途中暴露于黄病毒，包括WNV，而不是在北海道。
Recently, the distribution of Japanese encephalitis virus (JEV) and West Nile virus (WNV) has expanded into new territories. The invasion of WNV into Japan is of great concern. The migration of birds is suggested to be involved in the expanded distribution of these flaviviruses. In this study, 92 wild ducks-20 Anas poecilorhyncha (migratory breeders), 50 Anas platyrhynchos (undetermined), 16 Anas acuta (winter visitors), and 6 Anas penelope (winter visitors)-were captured in autumn of 2005 and 2006, in the central part of Hokkaido, a low JEV activity area. A seroepidemiologic analysis of flavivirus infections was conducted with 90% and 50% focus reduction neutralization tests (FRNT90 and FRNT50). Of the 92 serum samples, 1 (1.1%) and 5 (5.4%) tested positive for WNV-specific and JEV-specific antibodies, respectively, in the FRNT90, and 61 (66.3%) and 79 (85.9%) tested positive for WNV and JEV, respectively, in the FRNT50. These results indicate that wild ducks in this study had been exposed to flaviviruses. The results together with the recognized distribution of flaviviruses and migratory routes of individual duck species strongly suggested that the birds captured in this study had been exposed to flaviviruses, including WNV, on the flyway, not in Hokkaido.