MONOCLONAL NONSPECIFIC SUPPRESSOR FACTOR (MNSF) INHIBITS THE IL4 SECRETION BY BONE-MARROW-DERIVED MAST-CELL (BMMC)
MONOCLONAL NONSPECIFIC SUPPRESSOR FACTOR (MNSF) INHIBITS THE IL4 SECRETION BY BONE-MARROW-DERIVED MAST-CELL (BMMC)
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DOI：
10.1016/0014-5793(94)80423-0
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发表时间：
1994-02-21
期刊：
影响因子：
3.5
通讯作者：
TANIGAWA, Y
中科院分区：
文献类型：
--
作者：
NAKAMURA, M;XAVIER, RM;TANIGAWA, Y
本实验观察了单克隆非特异性抑制因子（MNSF）对抗DNP IgE单克隆抗体致敏的骨髓基质细胞（BMMC）分泌IL 4的影响。致敏的BMMC在10 ng/ml抗原活化时显示ILA分泌的最大增加。当加入MNSF（10 U）时，它显著抑制ILA分泌（82 +/- 3%）。MNSF还抑制肥大细胞系CFTL-15的IL 4分泌，但不抑制CFTL-12。北方印迹分析表明，当加入相同量的MNSF时，IL 4 mRNA表达降低，表明抑制是由于转录抑制的结果。
We investigated whether monoclonal non-specific suppressor factor (MNSF) has an effect on the IL4 secretion by BMMC sensitized with anti-DNP IgE monoclonal antibody. The sensitized BMMC showed a maximum increase of ILA secretion al 10 ng/ml antigen activation. When MNSF (10 U) was added, it remarkably suppressed the ILA secretion (82 +/- 3%). MNSF also suppressed the IL4 secretion by the mast cell line, CFTL-15, but not CFTL-12. Northern blot analysis showed that, when the same amounts of MNSF were added, IL4 mRNA expression was decreased, suggesting that the suppression results from the inhibition of a transcript.