Elevated A-to-I RNA editing in COVID-19 infected individuals.
Elevated A-to-I RNA editing in COVID-19 infected individuals.
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DOI：
10.1093/nargab/lqad092
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发表时间：
2023-12
影响因子：
4.6
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中科院分区：
文献类型：
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鉴于2019冠状病毒病（COVID-19）目前的状况是全球大流行病，深入了解该疾病的发展以及病毒如何影响其宿主是当务之急。腺苷（A）至肌苷（I）RNA编辑是由阿达尔酶家族催化的转录后修饰，其可以被认为是固有细胞防御机制的一部分，因为其以复杂的方式影响先天免疫应答。先前报道，各种病毒可以与宿主的阿达尔酶相互作用，导致病毒和宿主的表观遗传变化。在这里，我们分析了鼻咽拭子样本以及COVID-19感染个体的全血样本的RNA-seq，并显示与健康对照相比，COVID-19的全球RNA编辑活性显著升高。我们还检测到表现出更高编辑活性的特定编码位点。我们进一步表明，在疾病期间编辑活动的增加是暂时的，并在症状期后不久恢复到基线。这些显着的表观遗传变化可能有助于免疫系统反应并影响病毒感染后病例中出现的不良结果。
Given the current status of coronavirus disease 2019 (COVID-19) as a global pandemic, it is of high priority to gain a deeper understanding of the disease's development and how the virus impacts its host. Adenosine (A)-to-Inosine (I) RNA editing is a post-transcriptional modification, catalyzed by the ADAR family of enzymes, that can be considered part of the inherent cellular defense mechanism as it affects the innate immune response in a complex manner. It was previously reported that various viruses could interact with the host's ADAR enzymes, resulting in epigenetic changes both to the virus and the host. Here, we analyze RNA-seq of nasopharyngeal swab specimens as well as whole-blood samples of COVID-19 infected individuals and show a significant elevation in the global RNA editing activity in COVID-19 compared to healthy controls. We also detect specific coding sites that exhibit higher editing activity. We further show that the increment in editing activity during the disease is temporary and returns to baseline shortly after the symptomatic period. These significant epigenetic changes may contribute to the immune system response and affect adverse outcomes seen in post-viral cases.