Electrophoretic Heterogeneity of Lipopolysaccharides of Actinobacillus actinomycetemcomitans
Electrophoretic Heterogeneity of Lipopolysaccharides of Actinobacillus actinomycetemcomitans
复制标题
放线放线杆菌脂多糖的电泳异质性
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
C. Hoover
中科院分区：
文献类型：
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作者：
C. Hoover
通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）观察到的脂多糖（LPS）的电泳带型已被证明可用于研究LPS结构与毒力的相关性并作为流行病学标志物。本文采用SDS-聚丙烯酰胺凝胶电泳和脂多糖特异性银染技术对伴放线放线杆菌外膜组分和热酚水提取物中的脂多糖进行了分析。A.伴放线菌LPS。12株A.根据它们最快速迁移的LPS条带的相对迁移率，将作为所述三种血清型的代表的伴放线菌分配为LPS亚型。所有3种血清型a菌株（29523、aB 75和GA 3）、5种血清型B菌株中的2种（29524和SAC 11 A）以及4种血清型c菌株中的2种（aB 67和SAC 5A）被指定为LPS亚型I。其余三种血清型B菌株（29522、Y 4和JP 2）被指定为LPS亚型II，其余两种血清型C菌株（SAC 6A和SAC 12 A）被指定为LPS亚型III。LPS亚型分型可作为流行病学研究中血清分型的辅助或替代方法。
Electrophoretic banding patterns of lipopolysaccharides (LPS), as observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), have proved to be useful in studies relating LPS structure to virulence and as epidemiological markers. In this report, LPS of Actinobacillus actinomycetemcomitans from outer membrane fractions and hot phenol-water extracts were analyzed by SDS-PAGE and LPS-specific silver-staining techniques. Both intra- and inter-strain heterogeneity of A. actinomycetemcomitans LPS was observed. Twelve strains of A. actinomycetemcomitans, representative of the three described serotypes, were assigned to LPS subtypes based on the relative mobility of their most rapidly migrating LPS band. All three serotype a strains (29523, aB75, and GA3), two (29524 and SAC11A) of five serotype b strains, and two (aB67 and SAC5A) of four serotype c strains were assigned to LPS subtype I. The three remaining serotype b strains (29522, Y4, and JP2) were assigned to LPS subtype II, and the remaining two serotype c strains (SAC6A and SAC12A) were assigned to LPS subtype III. LPS subtyping may serve as an adjunct or alternative to serotyping in epidemiological studies.