Biological activities of the allatostatin family of peptides in the cockroach, Diploptera punctata, and potential interactions with receptors
Biological activities of the allatostatin family of peptides in the cockroach, Diploptera punctata, and potential interactions with receptors
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DOI：
10.1016/s0022-1910(99)00175-4
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发表时间：
2000-03-01
影响因子：
2.2
通讯作者：
Bendena, WG
中科院分区：
文献类型：
--
作者：
Tobe, SS;Zhang, JR;Bendena, WG
Allatostatin是抑制蟑螂（Diploptera punctata）中保幼激素产生的肽家族。很可能是allatostatin激素前体被加工以同时产生家族的所有13个成员。该家族的所有成员在抑制保幼激素产生的能力方面均显示出效力和有效性，尽管IC（50）和艾德（50）值显著不同，范围从Dippu-AST 2的最大值0.014 nM至Dippu-AST 1的最大值107 nM（艾德（50））。所有13种肽在组织和血淋巴中的可能存在表明它们可能协同作用以产生生理效应。我们使用了allatostatin的组合，包括所有13种肽的混合物，12（减去Dippu-AST 2）和11（减去Dippu-AST 2和5）以及高活性和低活性allatostatin的混合物在剂量-反应研究中，将肽（Dippu-AST 5加Dippu-AST 1或13）与肽（Dippu-AST 5加Dippu-AST 1或13）进行比较，以检查肽对生物活性的协同或累加效应的可能性。没有一种肽组合产生allatostatin之间协同相互作用的证据。然而，这些数据确实提供了对蟑螂中受体-配体相互作用的深入了解，并表明allatostatin通过与咽侧体中受体的不同亲和力相互作用的复杂混合物来调节JH生物合成。(C)2000爱思唯尔科技有限公司版权所有。
Allatostatins are a family of peptides that inhibit the production of:juvenile hormone in the cockroach, Diploptera punctata. It is likely that the allatostatin prohormone precursor is processed to give rise to all 13 members of the family simultaneously. All members of the family show potency and efficacy, in terms of their ability to inhibit juvenile hormone production, albeit with dramatically different IC(50) and ED(50) values, ranging from a maximum of 0.014 nM for Dippu-AST 2 to 107 nM for Dippu-AST 1 (ED(50)). The likely occurrence of all 13 peptides in tissues and in haemolymph suggests that they may act in concert to produce physiological effect We have employed combinations of the allatostatins, including a cocktail of all 13, 12 (minus Dippu-AST 2) and 11 (minus Dippu-AST 2 and 5) as well as mixtures of high and low activity allatostatins (Dippu-AST 5 plus either Dippu-AST 1 or 13) in dose-response studies to examine the possibility of synergistic or additive effects of the peptides on biological activity. None of the peptide combinations yielded evidence of synergistic interactions between allatostatins. However, the data do provide insight into receptor-ligand interactions in cockroaches and suggest the allatostatins regulate JH biosynthesis through a complex mix of differing affinity interactions with receptors in the corpora allata. (C) 2000 Elsevier Science Ltd. All rights reserved.