Detection of circulating epithelial cells after surgery for benign breast disease
Detection of circulating epithelial cells after surgery for benign breast disease
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DOI：
10.2165/00066982-200005010-00009
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发表时间：
2000-03-01
期刊：
影响因子：
--
通讯作者：
Dicarlo, RG
中科院分区：
文献类型：
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作者：
Crisan, D;Ruark, DS;Dicarlo, RG
背景：细胞角蛋白主要在上皮细胞及其恶性细胞中表达。细胞角蛋白信使 RNA (mRNA) 的超灵敏方法可以检测与上皮源性恶性肿瘤（包括乳腺癌）中的血行播散一致的罕见循环肿瘤细胞。术中肿瘤细胞脱落可能有助于这一过程；该假设基于手术操作过程中只有肿瘤细胞可以被动员的假设。方法和结果：本研究通过逆转录聚合酶链反应（RT-PCR）对 54 名因良性乳腺疾病接受切除活检的患者的术前和术后血液样本进行细胞角蛋白 19 mRNA 检测来解决这个问题； 22名健康志愿者代表对照组。在对照或术前样本中未发现细胞角蛋白 RT-PCR 阳性。 21例术后样本（39%）细胞角蛋白RT-PCR阳性。结论：这一结果表明良性上皮细胞在乳房手术过程中可以被动员；手术操作的这种影响在解释接受乳腺癌手术的患者外周血中细胞角蛋白 mRNA 的 RT-PCR 阳性时需要谨慎。
Background: Cytokeratins are predominantly expressed in epithelial cells and their malignant counterparts. Ultrasensitive methods for cytokeratin messenger RNAs (mRNAs) can detect rare circulating tumor cells consistent with hematogenous dissemination in epithelial-derived malignancies, including breast carcinomas. Intraoperative tumor-cell shedding may contribute to this process; this hypothesis is based on the assumption that only tumor cells can be mobilized during surgical manipulation.Methods and Results: The present study addresses this issue by using cytokeratin 19 mRNA detection by reverse transcription-polymerase chain reaction (RT-PCR) in preoperative and postoperative blood samples from 54 patients undergoing excisional biopsy for benign breast disease; 22 healthy volunteers represented the control group. No cytokeratin RT-PCR positivity was found in the control or pre operative samples. Cytokeratin RT-PCR positivity was found in 21 postoperative samples (39%).Conclusions: This finding shows that benign epithelial cells can be mobilized during breast surgery; this effect of surgical manipulation warrants caution in the interpretation of RT-PCR positivity for cytokeratin mRNA in the peripheral blood of patients undergoing surgery for breast cancer.