Linking molecular and population stress responses in Daphnia magna exposed to cadmium
Linking molecular and population stress responses in Daphnia magna exposed to cadmium
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DOI：
10.1021/es702469b
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发表时间：
2008-03-15
影响因子：
11.4
通讯作者：
Callaghan, Amanda
中科院分区：
文献类型：
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作者：
Connon, Richard;Hooper, Helen L.;Callaghan, Amanda
DNA 微阵列可用于测量环境应激反应。如果要预测环境影响，我们需要确定基因表达的改变是否会对个人和群体产生负面影响。创建了大型 cDNA 微阵列（14000 个点）来测量大型溞（最广泛使用的水生指示物种）对镉的分子应激反应，并将反应与种群增长率 (pgr) 联系起来。我们使用该阵列检测幼年 D. magna（24 小时龄）在暴露于对照和三种镉浓度（6、20 和 37 μg Cd2+ L-1）24 小时后基因转录的差异。在进一步接触 8 天后，估计了人群水平的压力反应。随着镉浓度在此范围内的增加，Pgr 近似呈线性负值。响应急性镉暴露的基因表达的微阵列谱开始提供 D. magna 分子反应的概述，特别是与生长和发育相关的分子反应。在响应基因中，29% 与新陈代谢（包括碳水化合物、脂肪和肽代谢）以及能量产生有关，31% 与转录/翻译有关，而 40% 的响应基因与细胞过程（如生长和换羽、离子运输和一般应激反应（包括氧化应激））相关。我们对大型水蚤微阵列的生产和应用表明，测量到的基因反应可以在逻辑上与镉等毒物对体细胞生长和发育的影响以及由此产生的 pgr 相关。
DNA microarrays can be used to measure environmental stress responses. If they are to be predictive of environmental impact, we need to determine if altered gene expression translates into negative impacts on individuals and populations. A large cDNA microarray (14000 spots) was created to measure molecular stress responses to cadmium in Daphnia magna,the most widely used aquatic indicator species, and relate responses to population growth rate (pgr). We used the array to detect differences in the transcription of genes in juvenile D. magna (24 h old) after 24 h exposure to a control and three cadmium concentrations (6, 20, and 37 mu g Cd2+ L-1). Stress responses at the population level were estimated following a further 8 days exposure. Pgr was approximately linear negative with increasing cadmium concentration over this range. The microarray profile of gene expression in response to acute cadmium exposure begins to provide an overview of the molecular responses of D. magna, especially in relation to growth and development. Of the responding genes, 29% were involved with metabolism including carbohydrate, fat and peptide metabolism, and energy production, 31% were involved with transcription/translation, while 40% of responding genes were associated with cellular processes like growth and moulting, ion transport, and general stress responses (which included oxidative stress). Our production and application of a large Daphnia magna microarray has shown that measured gene responses can be logically linked to the impact of a toxicant such as cadmium on somatic growth and development, and consequently pgr.