Inner nuclear membrane localization of epidermal growth factor receptor (EGFR) in spontaneous canine model of invasive micropapillary carcinoma of the mammary gland.
Inner nuclear membrane localization of epidermal growth factor receptor (EGFR) in spontaneous canine model of invasive micropapillary carcinoma of the mammary gland.
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DOI：
10.1016/j.prp.2015.11.017
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Cassali GD
中科院分区：
文献类型：
--
作者：
Rodrigues MA;Gamba CO;Faria JA;Ferreira Ê;Goes AM;Gomes DA;Cassali GD
表皮生长因子受体（EGFR）已被描述在原发性肿瘤的细胞核中。EGFR在细胞核的积累与DNA合成和细胞增殖有关，但细胞核EGFR的病理意义尚未完全了解。本研究的目的是探讨EGFR在犬乳腺浸润性微乳头状癌（IMPC）中的核定位。采用福尔马林和石蜡包埋组织的共聚焦免疫荧光法检测EGFR的亚细胞定位。我们的研究结果表明，EGFR共定位与内核被膜标志物，核纤层蛋白B1在IMPC。此外，EGFR在混合瘤型乳腺癌（CMT）中不定位于细胞核内或核膜内，这与比其他恶性组织学类型更好的预后相关。这一发现可能有助于作为IMPC治疗反应的预测生物标志物。
The epidermal growth factor receptor (EGFR) has been described in the nucleus of primary tumors. Accumulation of EGFR at the nucleus is linked to DNA synthesis and cell proliferation, but the pathological significance of nuclear EGFR is not completely understood. The aim of this study was to investigate the nuclear localization of EGFR in invasive micropapillary carcinoma (IMPC) that is an aggressive neoplasm of canine mammary gland. Confocal immunofluorescence of formalin and paraffin-embedded tissue was used to access the subcellular localization of EGFR. Our results demonstrated that EGFR co-localizes with the inner nuclear envelope marker, Lamin B1 in IMPC. Furthermore, EGFR was not localized within the nucleus or at the inner nuclear envelope membrane in mammary carcinoma in mixed tumor (CMT) that is associated with a better prognosis than other malignant histological types. This finding could be useful as a predictive biomarker of therapeutic response for IMPC.