Effect of Z-103 on TNB-induced colitis in rats.
Effect of Z-103 on TNB-induced colitis in rats.
复制标题
Z-103 对 TNB 诱导的大鼠结肠炎的影响。
作者：
T. Yoshikawa;T. Yamaguchi;N. Yoshida;H. Yamamoto;S. Kitazumi;S. Takahashi;Y. Naito;M. Kondo
Z-103是由锌离子和L-肌肽组成的螯合物。本研究探讨了Z-103对2，4，6-三硝基苯磺酸（TNB）所致大鼠结肠损伤的保护作用。通过在禁食48小时后向雄性Wistar大鼠（每只大鼠总体积为0.25ml）施用溶解在50%乙醇（120 mg/ml）中的TNB来诱导结肠炎症。在给予TNB后，以30 mg/kg/只大鼠的剂量给予Z-103 1周。第二组大鼠接受300 mg/kg的柳氮磺胺吡啶（SASP），第三组大鼠接受30 mg/kg的硫酸锌，持续1周。在TNB施用后1周评估结肠炎症。宏观和微观评价均表明，用Z-103、SASP和硫酸锌处理可降低TNB诱导的炎症反应。用Z-103、SASP和ZnSO 4治疗后，评分（根据肉眼病变从0到5分级）和结肠湿重（结肠远端8 cm）显著降低。在Z-103和SASP组中，TNB给药后结肠粘膜中硫代巴比妥酸反应性物质的增加受到抑制。这些结果表明Z-103与SASP一样有效对抗TNB诱导的结肠炎。
Z-103 is a chelate compound consisting of zinc ion and L-carnosine. In this study, we investigated the protective effect of Z-103 against colonic damage induced by 2,4,6-trinitrobenzene sulfonic acid (TNB) in rats. Colonic inflammation was induced by administering TNB dissolved in 50% ethanol (120 mg/ml) in male Wistar rats (total volume of 0.25 ml per rat) following a 48-hour fast. After the administration of TNB, Z-103 was given at a dose of 30 mg/kg per rat for 1 week. A second group of rats received sulfasalazine (SASP) at 300 mg/kg and a third group of rats received 30 mg/kg of ZnSO4 for 1 week. Colonic inflammation was assessed 1 week following TNB administration. Both macro- and microscopic evaluation showed that the inflammatory responses induced by TNB were reduced by treatment with Z-103, SASP and ZnSO4. The score (graded from 0 to 5 according to the macroscopic lesions) and colonic wet weight (distal 8 cm of the colon) were significantly decreased by treatment with Z-103, SASP and ZnSO4. The increase in thiobarbituric acid-reactive substances in the colonic mucosa following TNB administration were inhibited in the Z-103 and SASP groups. These results suggest that Z-103 is as effective against TNB-induced colitis as SASP.