Influences of microbubble diameter and ultrasonic parameters on in vitro sonothrombolysis efficacy.
Influences of microbubble diameter and ultrasonic parameters on in vitro sonothrombolysis efficacy.
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DOI：
10.1016/j.jvir.2012.08.019
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Culp WC
中科院分区：
文献类型：
--
作者：
Borrelli MJ;O'Brien WD Jr;Hamilton E;Oelze ML;Wu J;Bernock LJ;Tung S;Rokadia H;Culp WC
量化微泡(MB)大小、弹性和脉冲超声参数对体外超声溶栓(STBL：超声介导的溶栓)效果的影响。将直径为1μm或3μm的单分散微球置于脉冲超声(1 MHz或3 MHz)作用下，溶解兔血凝块。根据MB的大小和浓度、超声频率和强度、脉冲持续时间(PD)、脉冲重复频率(PRF)和占空比来测量STBL的疗效(血块质量损失)。在1 MHz频率下，使用3μm MB时，STBL值更有效，而在3 MHz时，使用1μm MB时，效果更好。当75%或更多的MB保持完好时，STBL值一般更高，尤其是对于3μmMB；将3μmMB的存活率从100 HZ提高到400 Hz时，STBL值的改善与3 MHzMB存活率的增加有关。然而，在3MHzSTBL值最大时，60%的1μmMB被破坏，这表明在这种情况下，STBL值可能需要相当大的MB崩塌。控制MB大小和弹性的能力允许利用广泛的超声参数(频率、强度等)。以产生所需的STBL水平。与之相比，3μm MBS(0.1W/cm~2)的最大短时照射强度(0.1W/cm~2)比1μm MBS(2.0W/cm~2)低20倍；体内短时照射存在潜在的安全性问题。使MB存活率最大化的超声参数产生了最大的短暂性白血病疗效；但在3MHz1μm MBS时，大多数MBS被破坏。
Quantify the effects of microbubble (MB) size, elasticity and pulsed ultrasonic parameters on in vitro sonothrombolysis (STBL: ultrasound-mediated thrombolysis) efficacy. Monodispersive MBs with diameters of 1 μm or 3 μm were exposed to pulsed ultrasound (1 MHz or 3 MHz) to lyse rabbit blood clots. STBL efficacy (clot mass loss) was measured as functions of MB size and concentration, ultrasonic frequency and intensity, pulse duration (PD), pulse repeat frequency (PRF) and duty factor. STBL at 1 MHz was more effective using 3 μm MBs and at 3 MHz using 1 μm MBs. STBL was generally more effective when 75% or more of MBs remained intact, especially for 3 μm MBs; and improving STBL by increasing PRF from 100 Hz to 400 Hz at 3 MHz was associated with increasing 3 μm MB survival. However, 60% of 1 μm MBs were destroyed during maximal STBL at 3 MHz, indicating that considerable MB collapse may be requisite for STBL under these conditions. The ability to control MB size and elasticity permits utilizing a wide range of ultrasound parameters (frequency, intensity, etc.) to produce desired levels of STBL. Comparable, maximal STBL efficacy was achieved at twenty-fold lower intensity with 3 μm MBs (0.1 W/cm2) than with 1 μm MBs (2.0 W/cm2); a potential safety issue for in vivo STBL. Ultrasound parameters that maximized MB survival yielded maximal STBL efficacy; except with 1 μm MBs at 3 MHz where most MBs were destroyed.
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影响因子：
8.3
作者：
Culp WC;Flores R;Brown AT;Lowery JD;Roberson PK;Hennings LJ;Woods SD;Hatton JH;Culp BC;Skinner RD;Borrelli MJ
通讯作者：
Borrelli MJ
影响因子：
7.5
作者：
Holland, Christy K.;Vaidya, Sampada S.;Shaw, George J.
通讯作者：
Shaw, George J.
影响因子：
3.4
作者：
DIAMOND, SL;ANAND, S
通讯作者：
ANAND, S
影响因子：
2.4
作者：
King, Daniel A.;Malloy, Michael J.;O'Brien, William D., Jr.
通讯作者：
O'Brien, William D., Jr.
DOI：
10.1109/58.19152
发表时间：
1989-03-01
影响因子：
3.6
作者：
HOLLAND, CK;APFEL, RE
通讯作者：
APFEL, RE