Immunization of female cynomolgus macaques with a synthetic epitope of sperm-specific lactate dehydrogenase results in high antibody titers but does not reduce fertility.
Immunization of female cynomolgus macaques with a synthetic epitope of sperm-specific lactate dehydrogenase results in high antibody titers but does not reduce fertility.
复制标题
用精子特异性乳酸脱氢酶的合成表位对雌性食蟹猴进行免疫会产生高抗体滴度，但不会降低生育能力。
DOI：
10.1002/mrd.10063
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Primakoff,PD
中科院分区：
文献类型：
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作者：
Tollner,TL;Overstreet,JW;Branciforte,D;Primakoff,PD
先前的研究报告称，使用源自精子特异性乳酸脱氢酶（LDH-C）的合成肽进行免疫接种的雌性狒狒的生育能力下降。在这项研究中，使用与破伤风毒素 (TT) 的 T 细胞表位缀合的相同肽序列 (bC5-19) 使用类似的方法对雌性食蟹猴进行免疫。 12 只雌性猴子用 Ribi MPL 佐剂载体递送的 bC5-19:TT 进行免疫，10 只对照雌性猴子仅注射佐剂载体。通过 ELISA 测量，治疗组中的所有 12 只雌性动物均产生了 LDH-C 特异性血清抗体，但在免疫动物的阴道液中未检测到抗 LDH-C 抗体。免疫后立即进行 4 个月的定时交配，十只免疫雌性中的五只怀孕了，十只对照雌性中的六只也怀孕了。来自怀孕和非怀孕 bC5-19:TT 免疫女性的抗血清在全精子提取物的蛋白质印迹上识别出 35 kDa 的单条带，并且来自同一血清的纯化 Igs 沿着透化精子鞭毛的主要部分定位。摩尔。重现。开发。 62: 257–264, 2002。© 2002 Wiley-Liss, Inc.
Previous studies have reported reduced fertility in female baboons immunized with a synthetic peptide derived from the sperm‐specific isozyme of lactate dehydrogenase (LDH‐C). In this study, a similar approach was used to immunize female cynomolgus macaques with the same peptide sequence (bC5‐19) conjugated to a T‐cell epitope from tetanus toxin (TT). Twelve female monkeys were immunized with bC5‐19:TT delivered with Ribi MPL adjuvant vehicle, and 10 control female monkeys were injected with the adjuvant vehicle only. All 12 females in the treatment group developed LDH‐C‐specific serum antibodies as measured by ELISA, but anti‐LDH‐C antibodies were not detected in vaginal fluids of the immunized animals. After 4 months of timed mating immediately following the immunizations, five of the ten immunized females became pregnant, as did six of the ten control females. Anti‐sera from both pregnant and nonpregnant bC5‐19:TT‐immunized females recognize a single band at 35 kDa on Western blots of whole sperm extracts, and purified Igs from the same sera localize along the principle piece of the flagellum of permeabilized sperm. Mol. Reprod. Dev. 62: 257–264, 2002. © 2002 Wiley‐Liss, Inc.