DISTRIBUTION OF D-1-DOPAMINE AND D-2-DOPAMINE RECEPTORS, AND DOPAMINE AND ITS METABOLITES IN THE HUMAN BRAIN
DISTRIBUTION OF D-1-DOPAMINE AND D-2-DOPAMINE RECEPTORS, AND DOPAMINE AND ITS METABOLITES IN THE HUMAN BRAIN
复制标题
DOI：
10.1038/sj.npp.1380111
复制
发表时间：
1994-12-01
影响因子：
7.6
通讯作者：
FARDE, L
中科院分区：
文献类型：
--
作者：
HALL, H;SEDVALL, G;FARDE, L
在受体结合试验和人死后全半球切片的放射自显影中，使用[H-3]SCH 23390和[H-3]雷氯必利在体外研究了D-1-多巴胺和D-2-多巴胺受体的密度和分布，以作为使用[C-11]SCH 23390和[C-11]雷氯必利进行PET研究的解剖学相关性。此外，多巴胺及其代谢产物的水平在不同的大脑区域通过HPLC测定。这两种多巴胺受体亚型，以及多巴胺，HVA和DOPAC，主要发现在基底神经节。非常高密度的D-1-多巴胺受体，特别是在内侧尾状核，而D-2-多巴胺受体均匀分布在整个尾状核。在尾状核和壳核中，D-1和D-2多巴胺受体的密度相似，而在几个边缘系统和新皮质区域中，D-1多巴胺受体的密度比D-2多巴胺受体的密度高4 - 7倍。放射自显影研究中的受体分布与使用[C-11]SCH 23390和[C-11]雷氯必利在活体人脑中证明的结果一致。
Densities and distribution of D-1-dopamine and D-2-dopamine receptors were investigated in vitro using [H-3]SCH 23390 and [H-3]raclopride in receptor binding assays and autoradiography on human post mortem whole hemisphere slices to serve as anatomical correlates to PET studies using [C-11]SCH 23390 and [C-11]raclopride. In addition, the levels of dopamine and its metabolites were determined by HPLC in various brain regions. Both dopamine receptor subtypes, as well as dopamine, HVA and DOPAC, were primarily found in the basal ganglia. Very high densities of D-1-dopamine receptors were found particularly in the medial caudate nucleus, whereas D-2-dopamine receptors were evenly distributed throughout the caudate. The densities of D-1- and D-2-dopamine receptors were similar in the caudate nucleus and the putamen, whereas there were 4 to 7 times higher densities of the D-1- than of the D-2-dopamine receptors in several limbic and neocortical regions. The receptor distribution in the autoradiographic study was consistent with that demonstrated in the living human brain using [C-11]SCH 23390 and [C-11]raclopride.