Variable ATPase composition of human tumor plasma membranes.
Variable ATPase composition of human tumor plasma membranes.
复制标题
人类肿瘤质膜的可变 ATP 酶组成。
DOI：
10.1016/0006-291x(81)90780-4
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发表时间：
1981
影响因子：
3.1
通讯作者：
Kaplan,NO
中科院分区：
文献类型：
--
作者：
Knowles,AF;Kaplan,NO
从几个可移植的人类肿瘤的纯化质膜表现出非常高的Mg 2+依赖的ATP酶活性。Mg ~（2+）依赖的ATP酶有三种类型：（1）哇巴因敏感的Na ~+，K ~+-ATP酶，它是肿瘤细胞质膜ATP酶的一种次要成分;（2）Mg ~（2+）激活的ATP酶，它是一种非特异性的核苷三磷酸酶;（3）ATP酶活性仅受Na ~+（或K ~+）刺激。在3例人黑色素瘤中，仅发现前两种活性，在1例星形细胞瘤和1例燕麦细胞癌中，发现所有三种活性，在这两种肿瘤中，质膜Mg ~（2+）-ATP酶也被ConA激活。并对这些ATP酶之间的关系进行了讨论。
Purified plasma membranes from several transplantable human tumors exhibit very high Mg2+-dependent ATPase activities. Three types of Mg2+-dependent ATPases can be demonstrated: (1) an ouabain sensitive Na+, K+-ATPase, which is a minor component of the tumor plasma membrane ATPase, (2) a Mg2+-activated ATPase, which is a non-specific nucleoside triphosphatase, and (3) an ATPase activity stimulated by Na+(or K+) alone. In three human melanomas, only the first two activities are found. In an astrocytoma and an oat cell carcinoma, all three activities are found. In the same two tumors, the plasma membrane Mg2+-ATPase is also stimulated by Con A. The relationship of these ATPases are discussed.