Correlation of p53 gene mutation and expression of P53 protein in cholangiocarcinoma
Correlation of p53 gene mutation and expression of P53 protein in cholangiocarcinoma
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DOI：
10.3748/wjg.v12.i29.4706
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发表时间：
2006-08-07
影响因子：
4.3
通讯作者：
Li, Shao-Jun
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Fang;Zhang, Hao;Li, Shao-Jun
目的：探讨肿瘤抑制基因p53在胆管癌中的突变特征，探讨p53基因突变与p53蛋白表达的相关性。方法：共收集36例未选择的冷冻胆管癌标本。结合患者临床参数，采用自动测序和免疫组化染色检测p53基因状态（外显子5-8）和p53蛋白。结果：36例患者中p53基因突变22例（61.1%）。36例患者中P53蛋白表达阳性19例（52.8%）。P53蛋白阳性表达与阴性表达在分化程度和侵袭程度上有显著差异。然而，突变和非突变之间的病理参数没有显著差异。结论：DNA序列分析评价p53基因的改变是比较准确的。P53蛋白的表达不能作为判断胆管癌预后的独立指标。(c) 2006年WIG出版社。版权所有。
AIM: To characterize the tumor suppressor gene p53 mutations and study the correlation of p53 gene mutation and the expression of P53 protein in cholangiocarcinoma.METHODS: A total of 36 unselected, frozen samples of cholangiocarcinoma were collected. p53 gene status(exon 5-8) and P53 protein were examined by automated sequencing and immunohistochemical staining, combined with the clinical parameters of patients.RESULTS: p53 gene mutations were found in 22 of 36 (61.1%) patients. Nineteen of 36 (52.8%) patients were positive for P53 protein expression. There were significant differences in extent of differentiation and invasion between the positive and negative expression of P53 protein. However, there were no significant differences in pathologic parameters between the mutations and non-mutations.CONCLUSION: The alterations of the p53 gene evaluated by DNA sequence analysis is relatively accurate. Expression of P53 protein could not act as an independent index to estimate the prognosis of cholangiocarcinoma. (c) 2006 The WIG Press. All rights reserved.