Visual phototransduction components in cephalopod chromatophores suggest dermal photoreception
Visual phototransduction components in cephalopod chromatophores suggest dermal photoreception
复制标题
DOI：
10.1242/jeb.117945
复制
发表时间：
2015-05-01
影响因子：
2.8
通讯作者：
Cronin, Thomas W.
中科院分区：
文献类型：
--
作者：
Kingston, Alexandra C. N.;Kuzirian, Alan M.;Cronin, Thomas W.
鲸类软体动物以其丰富多彩和动态的身体模式而闻名，这些模式是由皮肤组件与光线相互作用而产生的。这些可能包括虹膜细胞、白细胞、色素细胞和（在某些物种中）色素细胞。在这里，我们提出的分子证据表明，头足类色素细胞-小皮肤色素器官，反映各种颜色的光-是光敏的。RT-PCR揭示了编码视紫红质和视黄色素的转录本的存在下的视网膜和皮肤的鱿鱼Doryteuthis pealeii，乌贼乌贼和乌贼。In D. pealeii、Gqa和鱿鱼TRP通道转录本存在于视网膜和所有皮肤样品中。在分离的色素体的RNA提取物中也发现了视紫红质、视黄色素和Gqa转录本。pealeii真皮组织。免疫组织化学染色标记的视紫红质，视黄色素和Gqa蛋白质的几个色素细胞成分，包括色素细胞膜，桡肌纤维，鞘细胞。这是第一个证据表明头足类动物真皮组织，特别是色素细胞，可能拥有对光做出反应所需的分子组合。
Cephalopod mollusks are renowned for their colorful and dynamic body patterns, produced by an assemblage of skin components that interact with light. These may include iridophores, leucophores, chromatophores and (in some species) photophores. Here, we present molecular evidence suggesting that cephalopod chromatophores - small dermal pigmentary organs that reflect various colors of light - are photosensitive. RT-PCR revealed the presence of transcripts encoding rhodopsin and retinochrome within the retinas and skin of the squid Doryteuthis pealeii, and the cuttlefish Sepia officinalis and Sepia latimanus. In D. pealeii, Gqa and squid TRP channel transcripts were present in theretina and in all dermal samples. Rhodopsin, retinochrome and Gqa transcripts were also found in RNA extracts from dissociated chromatophores isolated from D. pealeii dermal tissues. Immunohistochemical staining labeled rhodopsin, retinochrome and Gqa proteins in several chromatophore components, including pigment cell membranes, radial muscle fibers, and sheath cells. This is the first evidence that cephalopod dermal tissues, and specifically chromatophores, may possess the requisite combination of molecules required to respond to light.