Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples
Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples
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DOI：
10.1128/aem.01152-07
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发表时间：
2007-09-01
影响因子：
4.4
通讯作者：
Inoue, Noborou
中科院分区：
文献类型：
--
作者：
Karanis, Panagiotis;Thekisoe, Oriel;Inoue, Noborou
建立并评价了环介导等温扩增(LAMP)检测环境和粪便样品中隐孢子虫的方法。这是首次将LAMP应用于隐孢子虫的检测。由于其特异性和简便性，该方法可以成为一种有用的诊断工具，用于隐孢子虫存在的表皮研究。
loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidermiologic studies of Cryptosporidium presence.