Xanthophyll cycle enzymes are members of the lipocalin family, the first identified from plants
Xanthophyll cycle enzymes are members of the lipocalin family, the first identified from plants
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DOI：
10.1074/jbc.273.25.15321
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发表时间：
1998-06-19
影响因子：
4.8
通讯作者：
Yamamoto, HY
中科院分区：
文献类型：
--
作者：
Bugos, RC;Hieber, AD;Yamamoto, HY
紫黄质去环氧化酶和玉米黄质环氧化酶催化植物叶黄素循环中类胡萝卜素中环氧基团的加入和去除。叶黄素循环与保护光合装置免受过度光照有关。报道了烟草和拟南芥中紫黄质去环氧酶的两个新序列。虽然成熟的蛋白质是保守的，但这些蛋白质的转运肽是不同的，与其他蛋白质的转运肽针对类囊体管腔。紫黄质去环氧化酶和玉米黄质环氧化酶的序列分析确定了叶黄素循环酶是脂钙蛋白家族的成员。脂钙蛋白家族是一种结合小疏水（亲脂）分子并共享8条p链形成桶状结构的保守三级结构的多样化蛋白质。这是首次报道在植物中发现脂钙蛋白。
Violaxanthin de-epoxidase and zeaxanthin epoxidase catalyze the addition and removal of epoxide groups in carotenoids of the xanthophyll cycle in plants. The xanthophyll cycle is implicated in protecting the photosynthetic apparatus from excessive light. Two new sequences for violaxanthin de-epoxidase from tobacco and Arabidopsis are described. Although the mature proteins are well conserved, the transit peptides of these proteins are divergent, in contrast to transit peptides from other proteins targeted to the thylakoid lumen. Sequence analyses of both violaxanthin de-epoxidase and zeaxanthin epoxidase establish the xanthophyll cycle enzymes as members of the lipocalin family of proteins. The lipocalin family is a diverse group of proteins that bind small hydrophobic (lipophilic) molecules and share a conserved tertiary structure of eight P-strands forming a barrel configuration. This is the first reported identification of lipocalin proteins in plants.