Cloning and characterization of Leishmania donovani telomeres
Cloning and characterization of Leishmania donovani telomeres
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DOI：
10.1006/expr.2000.4499
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发表时间：
2000-04-01
影响因子：
2.1
通讯作者：
Ramirez, JL
中科院分区：
文献类型：
--
作者：
Chiurillo, MA;Beck, AE;Ramirez, JL
我们在这里描述了克隆和序列表征的几个端粒的绝对末端从人类寄生虫利什曼原虫多诺瓦尼使用载体适配器协议。L. donovani端粒的3‘突出链以序列5’-GGTTAGGGT-OH 3'终止。该单链序列与串联重复的双链序列相邻，该双链序列由可变数目的六聚体重复5‘-TAGGGT-3’、可变数目的八聚体重复5‘-TGGTCATG-3’和单个62-bp序列组成。一些额外的，更多的染色体内部，非重复序列被发现邻近端粒序列。杂交分析表明，这些端粒邻近序列有的存在于所有L. donovani染色体上，有的在某些染色体亚群上更为丰富。有些对单个染色体来说是独一无二的。(C) 2000年学术出版社。
We describe here the cloning and sequence characterization of the absolute termini of several telomeres from the human parasite Leishmania donovani using a vector-adapter protocol. The 3' protruding strand of L. donovani telomeres terminates with the sequence 5'-GGTTAGGGT-OH 3'. This single-stranded sequence is adjacent to tandemly repeated blocks of double-stranded sequence consisting of variable numbers of the hexameric repeat 5'-TAGGGT-3', variable numbers of an octameric repeat 5'-TGGTCATG-3', and a single 62-bp sequence, in that order. A number of additional, more chromosome-internal, nonrepeated sequences were found adjacent to the telomere sequences. Hybridization analyses indicated that some of these telomere adjacent sequences are found on all L. donovani chromosomes, some are more abundant on certain subsets of chromosomes. and some are unique to individual chromosomes. (C) 2000 Academic Press.