QTL detection for rice grain quality traits using an interspecific backcross population derived from cultivated Asian (O-sativa L.) and African (O-glaberrima S.) rice
QTL detection for rice grain quality traits using an interspecific backcross population derived from cultivated Asian (O-sativa L.) and African (O-glaberrima S.) rice
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DOI：
10.1139/g04-029
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发表时间：
2004-08-01
期刊：
影响因子：
3.1
通讯作者：
McCouch, SR
中科院分区：
文献类型：
--
作者：
Li, JM;Xiao, JH;McCouch, SR
利用中国水稻杂交种常用的雄性不育系"V20 A“与来自马里的光果野生稻（Oryza glaberrima，IRGC No.103544）杂交获得的种间高代回交群体，分析了与稻米品质和形态相关的数量性状位点（QTL）。在中国长沙的田间条件下，对总共308个BC3F1杂交家系的16个籽粒相关性状进行了评估，并在康奈尔大学（纽约州伊萨卡）对相同的家系进行了RFLP和SSR标记分离评估。在6个染色体区域检测到与7个性状相关的11个QTL，其中优势等位基因来自O。glaberrima在8个位点。有利的O。glaberrima等位基因与粒形和外观的改善相关，导致粒长增加、较细粒的超亲变异和长宽比增加。其他位点的光秃稻等位基因与粗蛋白质含量和糙米产量的潜在提高相关。这些结果表明O. glaberrima可能有助于改善高产O.杂交品种
An interspecific advanced backcross population derived from a cross between Oryza sativa 'V20A' (a popular male-sterile line used in Chinese rice hybrids) and Oryza glaberrima (accession IRGC No. 103544 from Mali) was used to identify quantitative trait loci (QTL) associated with grain quality and grain morphology. A total of 308 BC3F1 hybrid families were evaluated for 16 grain-related traits under field conditions in Changsha, China, and the same families were evaluated for RFLP and SSR marker segregation at Cornell University (Ithaca, N.Y.). Eleven QTL associated with seven traits were detected in six chromosomal regions, with the favorable allele coming from O. glaberrima at eight loci. Favorable O. glaberrima alleles were associated with improvements in grain shape and appearance, resulting in an increase in kernel length, transgressive variation for thinner grains, and increased length to width ratio. Oryza glaberrima alleles at other loci were associated with potential improvements in crude protein content and brown rice yield. These results suggested that genes from O. glaberrima may be useful in improving specific grain quality characteristics in high-yielding O. sativa hybrid cultivars.