Specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to Plasmopara viticola
Specific adjustments in grapevine leaf proteome discriminating resistant and susceptible grapevine genotypes to Plasmopara viticola
复制标题
葡萄叶片蛋白质组的特异性调整可区分葡萄抗病基因型和易感基因型
DOI：
10.1016/j.jprot.2016.10.012
复制
发表时间：
2017-01-30
影响因子：
3.3
通讯作者：
Coelho, Ana Varela
中科院分区：
文献类型：
--
作者：
Figueiredo, Andreia;Martins, Joana;Coelho, Ana Varela
葡萄藤是影响作物生产的重要病害，严重影响产量。本研究旨在鉴定葡萄藤品种特异性的叶片蛋白质组调整，其允许区分对葡萄疫霉的抗性和敏感性（组成型（0 h）和接种后（6、12和24 h））。叶蛋白质组分析进行了2D差异凝胶电泳，然后通过质谱蛋白质鉴定。此外，我们分析了ROS的产生，抗氧化能力，脂质过氧化和基因表达。在接种葡萄疫霉之前，与光合作用和代谢相关的蛋白质允许区分抗性和感病的葡萄藤品种。接种后过氧化氢水平的增加，细胞氧化还原调节，ROS信号传导的建立和植物细胞死亡似乎是区分抗性基因型的关键点。脂质相关的信号事件，特别是有关茉莉酸似乎也发挥了重要作用，在建立电阻。这项研究的发现有助于更好地理解基因型特异性差异，这些差异是成功建立对霜霉病病原体的防御反应的原因。生物学意义：在这里，我们第一次提出了葡萄品种特异性的叶片蛋白质组调整，允许对葡萄疫霉的抗性和易感性之间的歧视（组成型（0小时）和接种后（6、12和24小时）。我们已经强调，接种后，主要因素区分敏感葡萄藤品种的电阻是建立有效的ROS信号与脂质相关的信号事件，特别是有关茉莉酸。一般认为，受活体营养型病原体感染的植物抑制JA介导的反应，然而最近的证据表明，茉莉酸信号通路在葡萄藤抗霜霉病中起作用。我们的研究结果证实了这些证据，并强调了脂质信号传导对霜霉病病原体有效抗性反应的重要性。(C)2016爱思唯尔B.V.保留所有权利。
Grapevine downy mildew is an important disease affecting crop production leading to severe yield losses. This study aims to identify the grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards P. viticola (constitutive (0 h) and in after inoculation (6, 12 and 24 h). Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry. In addition, we analysed ROS production, antioxidant capacity, lipid peroxidation and gene expression. Proteins related to photosynthesis and metabolism allowed the discrimination of resistant and susceptible grapevine cultivars prior to P. viticola inoculation. Following inoculation increase of hydrogen peroxide levels, cellular redox regulation, establishment of ROS signalling and plant cell death seem to be key points differentiating the resistant genotype. Lipid associated signalling events, particularly related to jasmonates appear also to play a major role in the establishment of resistance. The findings from this study contribute to a better understanding of genotype-specific differences that account for a successful establishment of a defence response to the downy mildew pathogen.Biological significance: Here, we present for the first time grapevine cultivar-specific adjustments of leaf proteome that allow the discrimination between resistance and susceptibility towards P. viticola (constitutive (0 h) and in after inoculation (6, 12 and 24 h). We have highlighted that, following inoculation, the major factors differentiating the resistant from the susceptible grapevine cultivars are the establishment of effective ROS signalling together with lipid-associated signalling events, particularly related to jasmonates. It is believed that plants infected with biotrophic pathogens suppress JA-mediated responses, however recent evidences shown that jasmonic acid signalling pathway in grapevine resistance against Plasmopara viticola. Our results corroborate those evidences and highlight the importance of lipid- signalling for an effective resistance response against the downy mildew pathogen. (C) 2016 Elsevier B.V. All rights reserved.